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Literature summary extracted from

  • Benedict, C.R.; Madhavan, S.; Greenblatt, G.A.; Venkatachalam, K.V.; Foster, M.A.
    The Enzymatic Synthesis of Rubber Polymer in Parthenium argentatum Gray (1989), Plant Physiol., 92, 816-821.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.20 glutathione required for maximal activity Parthenium argentatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.20 0.083
-
 isopentenyl diphosphate
-
 Parthenium argentatum
2.5.1.20 0.096
-
 dimethylallyl diphosphate
-
 Parthenium argentatum
2.5.1.20 0.52
-
 Mg2+
-
 Parthenium argentatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.20 Mg2+ required for activity Parthenium argentatum
2.5.1.20 Mg2+ Km: 0.52 mM Parthenium argentatum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.20 Parthenium argentatum
-
 var N565II
-
2.5.1.20 Parthenium argentatum
-
 guayule
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.20 dimethylallyl diphosphate + isopentenyl diphosphate
-
 Parthenium argentatum (E,E)-farnesyl diphosphate + (E)-geranyl diphosphate
-
 ?
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene allylic diphosphate Parthenium argentatum diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit the peak molecular weight of the radioactive polymer increases from 70000 Da in 15 min to 750000 Da in 3 h, the weight average molecular weight of the polymer synthesized over a 3 h period is 117000 Da compared to 149000 Da of the natural rubber polymer ?
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene of rubber particles Parthenium argentatum diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit the peak molecular weight of the radioactive polymer increases from 70000 Da in 15 min to 750000 Da in 3 h, the weight average molecular weight of the polymer synthesized over a 3 h period is 117000 Da compared to 149000 Da of the natural rubber polymer ?