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Literature summary extracted from

  • Sagami, H.; Ogura, K.
    Geranylpyrophosphate synthetase-geranylgeranylpyrophosphate synthetase from Micrococcus luteus (1985), Methods Enzymol., 110, 188-192.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.29 Triton X-100 stimulates farnesyl-transferring activity, inhibits dimethylallyl-transferring activity Micrococcus luteus
2.5.1.29 Tween 80 stimulates Micrococcus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.29 diphosphate
-
 Micrococcus luteus
2.5.1.29 farnesyl diphosphate
-
 Micrococcus luteus
2.5.1.29 Triton X-100 stimulates farnesyl-transferring activity, inhibits dimethylallyl-transferring activity Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.29 Mg2+ required for activity Micrococcus luteus
2.5.1.29 Mg2+ optimal activation of farnesyl-transferring activity at 1 mM, optimal activation of dimethylallyl-transferring activity at 3 mM Micrococcus luteus
2.5.1.29 Mn2+ slight activation Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 70000
-
 gel filtration Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Micrococcus luteus
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Micrococcus luteus
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Micrococcus luteus
-
 the activities of EC 2.5.1.1 and EC 2.5.1.29 may be a mixture of 2 enzymes or a single enzyme with two independent catalytic sites, see EC 2.5.1.1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29
-
 Micrococcus luteus

Storage Stability

EC Number Storage Stability Organism
2.5.1.29 -20°C, at least 1 week, no loss of activity Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (E)-3-methyl-2-dodecenyl diphosphate + isopentenyl diphosphate 45% of the activity with farnesyl diphosphate Micrococcus luteus (2E,5E)-3,6-dimethylpentadeca-2,5-dien-1-yl diphosphate + diphosphate
-
 ?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 E-3-methyl-2-undecenyl diphosphate + isopentenyl diphosphate 53% of the activity with farnesyl diphosphate Micrococcus luteus ?
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Micrococcus luteus geranylgeranyl diphosphate + 2 diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.29 7.7
-
-
 Micrococcus luteus