EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.10 | expression of wild-type, S82F, S82Y, S82W, L83F, L83Y, I84F and I84Y mutant enzymes in Escherichia coli | Geobacillus stearothermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.10 | I84F | 7.2% of wild-type activity with dimethylallyl diphosphate | Geobacillus stearothermophilus |
2.5.1.10 | I84Y | similiar activity as wild-type | Geobacillus stearothermophilus |
2.5.1.10 | L83F | similiar activity as wild-type | Geobacillus stearothermophilus |
2.5.1.10 | L83Y | similiar activity as wild-type | Geobacillus stearothermophilus |
2.5.1.10 | S82F | produces exclusively geranyl diphosphate, i.e. the mutant enzyme has changed from a farnesyl diphosphate synthase to a geranyl diphosphate synthase | Geobacillus stearothermophilus |
2.5.1.10 | S82W | no prenyl transferase activity | Geobacillus stearothermophilus |
2.5.1.10 | S82Y | similiar activity as wild-type | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.10 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.10 | dimethylallyl diphosphate + 2 isopentenyl diphosphate | no activity with farnesyl diphosphate | Geobacillus stearothermophilus | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? |