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Literature summary extracted from

  • Tholl, D.; Croteau, R.; Gershenzon, J.
    Partial purification and characterization of the short-chain prenyltransferases, gernayl diphospate synthase and farnesyl diphosphate synthase, from Abies grandis (grand fir) (2001), Arch. Biochem. Biophys., 386, 233-242.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.10 additional information not activated by Tween 20, Triton X-100, Chaps or Nonidet P-40 Abies grandis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 diphosphate 0.66 mM, 50% inhibition Abies grandis
2.5.1.10 farnesyl diphosphate 0.06 mM, 50% inhibition Abies grandis
2.5.1.10 K+ 500 mM, 63% inhibition in the presence of MgCl2 Abies grandis
2.5.1.10 Mn2+ strong inhibition above 0.1 mM, activation below Abies grandis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.10 0.0018
-
 geranyl diphosphate
-
 Abies grandis
2.5.1.10 0.009
-
 dimethylallyl diphosphate
-
 Abies grandis
2.5.1.10 0.0153
-
 isopentenyl diphosphate
-
 Abies grandis
2.5.1.10 0.0445
-
 Mn2+
-
 Abies grandis
2.5.1.10 0.0712
-
 Mg2+
-
 Abies grandis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.10 Ca2+ 18% of Mg2+ activation at 0.5 mM, inhibition above Abies grandis
2.5.1.10 Co2+ 78% of Mg2+ activation at 1 mM Abies grandis
2.5.1.10 Mg2+ maximal activity at 1 mM Abies grandis
2.5.1.10 Mg2+ required for activity Abies grandis
2.5.1.10 Mn2+ can replace Mg2+, maximal activity at 0.1 mM, inhibition above Abies grandis
2.5.1.10 Ni2+ 10% of Mg2+ activation at 0.5 mM, inhibition above Abies grandis
2.5.1.10 Zn2+ 91% of Mg2+ activation at 0.5 mM, almost complete inhibition at 5 mM Abies grandis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.10 110000
-
 gel filtration Abies grandis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Abies grandis
-
 grand fir
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 DEAE-Sepharose, Mono Q, phenyl-Sepharose Abies grandis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.10 sapling
-
 Abies grandis
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.10 -80°C, 3 months, no loss of activity Abies grandis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
 Abies grandis (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Abies grandis diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.10 7.5 8
-
 Abies grandis