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Literature summary extracted from

  • Koyama, T.; Obata, S.; Saito, K.; Takeshita-Koike, A.; Ogura, K.
    Structural and functional roles of the cysteine residues of Bacillus stearothermophilus farnesyl diphosphate synthase (1994), Biochemistry, 33, 12644-12648.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10 overexpression of C73F, C289F, C73S, C289S and C73/C289S mutant enzyme in Escherichia coli Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.10 C289F approx. 1/13 of wild-type activity at 55°C Geobacillus stearothermophilus
2.5.1.10 C289S similar activity like wild-type Geobacillus stearothermophilus
2.5.1.10 C73F heat sensitive mutant Geobacillus stearothermophilus
2.5.1.10 C73S similar activity like wild-type Geobacillus stearothermophilus
2.5.1.10 C73S/C289S similar activity like wild-type Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 p-chloromercuribenzoic acid 0.15 mM, 882% inhibition of wild-type farnesyl diphosphate synthase, 11% inhibition of C73S/C289S mutant enzyme Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.10 4.7
-
isopentenyl diphosphate wild-type enzyme at 45°C Geobacillus stearothermophilus
2.5.1.10 4.8
-
isopentenyl diphosphate C73F mutant enzyme at 45°C Geobacillus stearothermophilus
2.5.1.10 4.9
-
geranyl diphosphate C73F mutant enzyme at 45°C Geobacillus stearothermophilus
2.5.1.10 5.6
-
geranyl diphosphate C73S/C289S mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 5.8
-
geranyl diphosphate wild-type enzyme at 45°C Geobacillus stearothermophilus
2.5.1.10 6.4
-
geranyl diphosphate C289S mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 7.3
-
geranyl diphosphate C73F mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 7.5
-
geranyl diphosphate C289F mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 8.4
-
geranyl diphosphate wild-type enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 11.6
-
isopentenyl diphosphate C73F mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 13
-
isopentenyl diphosphate wild-type enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 13.3
-
isopentenyl diphosphate C73S/C289S mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 14.3
-
isopentenyl diphosphate C289S mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 130
-
isopentenyl diphosphate C289F mutant enzyme at 55°C Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Geobacillus stearothermophilus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.5.1.10 enzyme seems to be susceptible to oxidation when treated with Cu2+, forming an intrasubunit disulfide bond rather than intersubunit bonds Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.10 0.35
-
C289F mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 1.3
-
C73F mutant enzyme at 45°C Geobacillus stearothermophilus
2.5.1.10 2.61
-
wild-type enzyme at 45°C Geobacillus stearothermophilus
2.5.1.10 3.94
-
C289S mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 3.97
-
C73S/C289S mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 4.3
-
C73F mutant enzyme at 55°C Geobacillus stearothermophilus
2.5.1.10 4.69
-
wild-type enzyme at 55°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
Geobacillus stearothermophilus (E,E)-farnesyl diphosphate + 2 diphosphate
-
?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
Geobacillus stearothermophilus diphosphate + farnesyl diphosphate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.10 55
-
wild-type: stable for 30 min, C73F mutant: complete loss of activity after 20 min Geobacillus stearothermophilus