EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.10 | overexpression of C73F, C289F, C73S, C289S and C73/C289S mutant enzyme in Escherichia coli | Geobacillus stearothermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.10 | C289F | approx. 1/13 of wild-type activity at 55°C | Geobacillus stearothermophilus |
2.5.1.10 | C289S | similar activity like wild-type | Geobacillus stearothermophilus |
2.5.1.10 | C73F | heat sensitive mutant | Geobacillus stearothermophilus |
2.5.1.10 | C73S | similar activity like wild-type | Geobacillus stearothermophilus |
2.5.1.10 | C73S/C289S | similar activity like wild-type | Geobacillus stearothermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.10 | p-chloromercuribenzoic acid | 0.15 mM, 882% inhibition of wild-type farnesyl diphosphate synthase, 11% inhibition of C73S/C289S mutant enzyme | Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.10 | 4.7 | - |
isopentenyl diphosphate | wild-type enzyme at 45°C | Geobacillus stearothermophilus | |
2.5.1.10 | 4.8 | - |
isopentenyl diphosphate | C73F mutant enzyme at 45°C | Geobacillus stearothermophilus | |
2.5.1.10 | 4.9 | - |
geranyl diphosphate | C73F mutant enzyme at 45°C | Geobacillus stearothermophilus | |
2.5.1.10 | 5.6 | - |
geranyl diphosphate | C73S/C289S mutant enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 5.8 | - |
geranyl diphosphate | wild-type enzyme at 45°C | Geobacillus stearothermophilus | |
2.5.1.10 | 6.4 | - |
geranyl diphosphate | C289S mutant enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 7.3 | - |
geranyl diphosphate | C73F mutant enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 7.5 | - |
geranyl diphosphate | C289F mutant enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 8.4 | - |
geranyl diphosphate | wild-type enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 11.6 | - |
isopentenyl diphosphate | C73F mutant enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 13 | - |
isopentenyl diphosphate | wild-type enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 13.3 | - |
isopentenyl diphosphate | C73S/C289S mutant enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 14.3 | - |
isopentenyl diphosphate | C289S mutant enzyme at 55°C | Geobacillus stearothermophilus | |
2.5.1.10 | 130 | - |
isopentenyl diphosphate | C289F mutant enzyme at 55°C | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.10 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
2.5.1.10 | enzyme seems to be susceptible to oxidation when treated with Cu2+, forming an intrasubunit disulfide bond rather than intersubunit bonds | Geobacillus stearothermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.10 | 0.35 | - |
C289F mutant enzyme at 55°C | Geobacillus stearothermophilus |
2.5.1.10 | 1.3 | - |
C73F mutant enzyme at 45°C | Geobacillus stearothermophilus |
2.5.1.10 | 2.61 | - |
wild-type enzyme at 45°C | Geobacillus stearothermophilus |
2.5.1.10 | 3.94 | - |
C289S mutant enzyme at 55°C | Geobacillus stearothermophilus |
2.5.1.10 | 3.97 | - |
C73S/C289S mutant enzyme at 55°C | Geobacillus stearothermophilus |
2.5.1.10 | 4.3 | - |
C73F mutant enzyme at 55°C | Geobacillus stearothermophilus |
2.5.1.10 | 4.69 | - |
wild-type enzyme at 55°C | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.10 | dimethylallyl diphosphate + 2 isopentenyl diphosphate | - |
Geobacillus stearothermophilus | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
2.5.1.10 | geranyl diphosphate + isopentenyl diphosphate | - |
Geobacillus stearothermophilus | diphosphate + farnesyl diphosphate | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.10 | 55 | - |
wild-type: stable for 30 min, C73F mutant: complete loss of activity after 20 min | Geobacillus stearothermophilus |