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Literature summary extracted from

  • Green, T.R.; West, C.A.
    Purification and characterization of two forms of geranyl transferase from Ricinus communis (1974), Biochemistry, 13, 4720-4729.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 iodoacetamide 8 mM, 33% inhibition Ricinus communis
2.5.1.10 Mn2+ activates at 0.25-0.50 mM, inhibition above Ricinus communis
2.5.1.10 additional information neither iodoacetamide nor N-ethylmaleimide inhibits at concentration less than 5 mM Ricinus communis
2.5.1.10 N-ethylmaleimide 8 mM, 54% inhibition Ricinus communis
2.5.1.10 p-hydroxymercuribenzoate 1 mM, 25% inhibition Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.10 0.0045
-
 dimethylallyl diphosphate 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration Ricinus communis
2.5.1.10 0.0059
-
 dimethylallyl diphosphate 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration Ricinus communis
2.5.1.10 0.032
-
 isopentenyl diphosphate 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration Ricinus communis
2.5.1.10 0.036
-
 geranyl diphosphate 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration Ricinus communis
2.5.1.10 0.039
-
 geranyl diphosphate 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration Ricinus communis
2.5.1.10 0.048
-
 isopentenyl diphosphate 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration Ricinus communis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.10 Mg2+ required for activity Ricinus communis
2.5.1.10 Mg2+ maximum activity at 1-2 mM Ricinus communis
2.5.1.10 Mn2+ activation at 0.25-0.50 mM, inhibition above Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.10 56000
-
 gel filtration, enzyme I, protein concentration 0.02 mg/ml Ricinus communis
2.5.1.10 60000
-
 gel filtration, enzyme II, protein concentration 0.02 mg/ml Ricinus communis
2.5.1.10 72500
-
 gel filtration, protein concentration 25 mg/ml Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Ricinus communis
-
 isoenzyme I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10
-
 Ricinus communis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.10 0.04
-
 isoenzyme II Ricinus communis
2.5.1.10 0.06
-
 isoenzyme I Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
 Ricinus communis (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Ricinus communis diphosphate + farnesyl diphosphate E)-farnesyl diphosphate ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.10 6.8
-
-
 Ricinus communis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.10 5.5 8.5 approx. 45% of maximal activity at pH 5.5, approx. 60% of activity maximum at pH 8.5, emzyme I Ricinus communis