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Literature summary extracted from

  • Takahashi, I.; Ogura, K.
    Farnesyl pyrophosphate synthetase from Bacillus subtilis (1981), J. Biochem., 89, 1581-1587.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.10 1,4-dithiothreitol 2 mM, 1.6fold stimulation Bacillus subtilis
2.5.1.10 2-mercaptoethanol 10 mM, 1.2fold stimulation Bacillus subtilis
2.5.1.10 NH4+ 3fold stimulation with geranyl diphosphate as substrate Bacillus subtilis
2.5.1.10 NH4+ 5fold stimulation with dimethylallyl diphosphate as substrate Bacillus subtilis
2.5.1.10 Triton X-100 0.5%, 3fold activation Bacillus subtilis
2.5.1.10 Tween 80 0.5%, 2.3fold activation Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 bacitracin
-
 Bacillus subtilis
2.5.1.10 Ca2+ 5 mM, 80% inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.10 0.018
-
 geranyl diphosphate
-
 Bacillus subtilis
2.5.1.10 0.05
-
 dimethylallyl diphosphate
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.10 K+ stimulates with geranyl diphosphate as substrate Bacillus subtilis
2.5.1.10 Mg2+ required for activity Bacillus subtilis
2.5.1.10 Mn2+ can partially replace Mg2+, less effective Bacillus subtilis
2.5.1.10 additional information not activated by Na+ or Li+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.10 67000
-
 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 ammonium sulfate, Sephadex G-100, hydroxylapatite, DEAE-Sephadex A-50 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.10 0.0012
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.5.1.10 -20°C, at least 1 week, no loss of activity Bacillus subtilis
2.5.1.10 4°C, 5 days, 36% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
 Bacillus subtilis (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis diphosphate + farnesyl diphosphate E)-farnesyl diphosphate ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.10 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.10 8.5
-
-
 Bacillus subtilis