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Literature summary extracted from

  • Egan, A.F.; Gibson, F.
    Anthranilate synthase-anthranilate 5-phosphoribosyl 1-pyrophosphate phosphoribosyltransferase from Aerobacter aerogenes (1972), Biochem. J., 130, 847-859.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.4.2.18 dilution inactivates Klebsiella aerogenes
2.4.2.18 freezing and thawing inactivates Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.18 L-tryptophan
-
 Escherichia coli
2.4.2.18 L-tryptophan when phosphoribosyltransferase is not an aggregate with anthranilate synthase, it is not subject to tryptophan inhibition, inhibitor site is on the anthranilate synthase component Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.18 Mg2+ required Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.18 90000
-
 tryptophan auxotroph with no anthranilate synthase activity, sucrose density gradient sedimentation Klebsiella aerogenes
2.4.2.18 170000
-
 sucrose density gradient sedimentation, complex with anthranilate synthase activity Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Escherichia coli enzyme of tryptophan biosynthesis N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Klebsiella aerogenes enzyme of tryptophan biosynthesis N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.18 Escherichia coli
-
 K-12
-
2.4.2.18 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.18 partial, enzyme complex Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.18 734
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.4.2.18 -15°C, crude extract is stable for 2 years Klebsiella aerogenes
2.4.2.18 4°C, 10% loss of activity after 2 months Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Klebsiella aerogenes N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate enzyme of tryptophan biosynthesis Escherichia coli N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate enzyme of tryptophan biosynthesis Klebsiella aerogenes N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.18 More in some organisms, this enzyme is part of a multifunctional protein together with one or more other components of the system for biosynthesis of tryptophan: EC 4.1.1.48, EC 4.1.3.27, EC 4.2.1.20, EC 5.3.1.24 Klebsiella aerogenes
2.4.2.18 More anthranilate synthase-phosphoribosylanthranilate transferase complex exists in Citrobacter species in all other bacteria examined phosphoribosylanthranilate transferase and anthranilate synthase are separate enzyme molecules Escherichia coli
2.4.2.18 More anthranilate synthase-phosphoribosylanthranilate transferase complex exists in Citrobacter species in all other bacteria examined phosphoribosylanthranilate transferase and anthranilate synthase are separate enzyme molecules Klebsiella aerogenes

Synonyms

EC Number Synonyms Comment Organism
2.4.2.18 More in some organisms, this enzyme is part of a multifunctional protein together with one or more other components of the system for biosynthesis of tryptophan (EC 4.1.1.48, EC 4.1.3.27, EC 4.2.1.20, EC 5.3.1.24) Klebsiella aerogenes