EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.14 | D-fructose-1-phosphate | slight activation | Spinacia oleracea | |
2.4.1.14 | D-glucose-1-phosphate | slight activation | Spinacia oleracea | |
2.4.1.14 | glucose-6-phosphate | - |
Spinacia oleracea |
EC Number | General Stability | Organism |
---|---|---|
2.4.1.14 | 20% ethylene glycol and 0.2 M KCl stabilize activity | Spinacia oleracea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.14 | diphosphate | slight inhibition | Spinacia oleracea | |
2.4.1.14 | NaF | strong inhibition at 20 mM | Spinacia oleracea | |
2.4.1.14 | phosphate | in absence of glucose-6-phosphate, no inhibition at pH 5.5, inhibitory at alkaline pH | Spinacia oleracea | |
2.4.1.14 | phosphoenolpyruvate | slight inhibition | Spinacia oleracea | |
2.4.1.14 | UDP | - |
Spinacia oleracea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.14 | 0.7 | - |
D-fructose 6-phosphate | in presence of 5 mM glucose 6-phosphate | Spinacia oleracea | |
2.4.1.14 | 3.2 | - |
D-fructose 6-phosphate | in the absence of glucose 6-phosphate | Spinacia oleracea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.14 | 460000 | - |
gel filtration | Spinacia oleracea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.14 | UDP-alpha-D-glucose + D-fructose 6-phosphate | Spinacia oleracea | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.14 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.14 | gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity | Spinacia oleracea |
2.4.1.14 | partial | Spinacia oleracea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.14 | leaf | - |
Spinacia oleracea | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.14 | UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
2.4.1.14 | UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.14 | 7.5 | - |
broad optimum | Spinacia oleracea |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.14 | 5.5 | 9 | - |
Spinacia oleracea |