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Literature summary extracted from

  • Doehlert, D.C.; Huber, S.C.
    Regulation of spinach leaf sucrose phosphate synthase by glucose-6-phosphate, inorganic phosphate and pH (1983), Plant Physiol., 73, 989-994.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.14 D-fructose-1-phosphate slight activation Spinacia oleracea
2.4.1.14 D-glucose-1-phosphate slight activation Spinacia oleracea
2.4.1.14 glucose-6-phosphate
-
Spinacia oleracea

General Stability

EC Number General Stability Organism
2.4.1.14 20% ethylene glycol and 0.2 M KCl stabilize activity Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 diphosphate slight inhibition Spinacia oleracea
2.4.1.14 NaF strong inhibition at 20 mM Spinacia oleracea
2.4.1.14 phosphate in absence of glucose-6-phosphate, no inhibition at pH 5.5, inhibitory at alkaline pH Spinacia oleracea
2.4.1.14 phosphoenolpyruvate slight inhibition Spinacia oleracea
2.4.1.14 UDP
-
Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 0.7
-
D-fructose 6-phosphate in presence of 5 mM glucose 6-phosphate Spinacia oleracea
2.4.1.14 3.2
-
D-fructose 6-phosphate in the absence of glucose 6-phosphate Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 460000
-
gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity Spinacia oleracea
2.4.1.14 partial Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.14 leaf
-
Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Spinacia oleracea UDP + sucrose 6-phosphate
-
?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.14 7.5
-
broad optimum Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.14 5.5 9
-
Spinacia oleracea