Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sprenger, N.; Keller, F.
    Allocation of raffinose family oligosaccharides to transport and storage pools in Ajuga reptans: the roles of two distinct galactinol synthases (2000), Plant J., 21, 249-258.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.123 additional information cold-inducible GolS-1 and 2 genes Ajuga reptans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.123 cold-inducible GolS-1 and -2 genes encode 2 distinct galactinol synthases, cloning and sequencing of the GolS-1 and -2 genes, deduced amino acid sequences, expression of GolS-1 cDNA in Escherichia coli as functional enzyme Ajuga reptans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol Ajuga reptans galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
?
2.4.1.123 UDP-galactose + myo-inositol Ajuga reptans first committed enzyme in biosynthesis of raffinose family oligosaccharides, which play a role in plant stress tolerance UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.123 Ajuga reptans Q9XGN3 GolS-2, 2 isoforms: GolS-1 and GolS-2
-
2.4.1.123 Ajuga reptans Q9XGN4 GolS-1, 2 isoforms: GolS-1 and GolS-2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.123 phosphoprotein putative serine phosphorylation site at GolS-1 codon position 263 Ajuga reptans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.123 leaf source and sink leaves, GolS-1 is source leaf-specific, equally weak expression of GolS-2 in all leaf types Ajuga reptans
-
2.4.1.123 mesophyll GolS-1 is primarily expressed in the mesophyll, the site of raffinose family oligosaccharides storage Ajuga reptans
-
2.4.1.123 phloem GolS-2 is primarily expressed in the phloem-associated intermediary cells known for their role in raffinose family oligosaccharides phloem loading Ajuga reptans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol reaction is probably reversible Ajuga reptans UDP + 1-O-alpha-D-galactosyl-D-myo-inositol identical with galactinol r
2.4.1.123 UDP-galactose + myo-inositol galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction Ajuga reptans UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
?
2.4.1.123 UDP-galactose + myo-inositol first committed enzyme in biosynthesis of raffinose family oligosaccharides, which play a role in plant stress tolerance Ajuga reptans UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.123 30
-
assay at Ajuga reptans