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Literature summary extracted from

  • Durchschlag, H.; Biedermann, G.; Eggerer, H.
    Large-scale purification and some properties of malate synthase from bakers yeast (1981), Eur. J. Biochem., 114, 255-262.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.9 glycolate
-
 Saccharomyces cerevisiae
2.3.3.9 oxalate
-
 Saccharomyces cerevisiae
2.3.3.9 oxaloacetate
-
 Saccharomyces cerevisiae
2.3.3.9 pyruvate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.083
-
 acetyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.9 0.1
-
 glyoxylate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 Mg2+ binding of acetyl-CoA to the synthase is independent of Mg2+ but that of glyoxylate is strictly dependent on the presence of Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 66000
-
 3 * 66000, SDS-PAGE Saccharomyces cerevisiae
2.3.3.9 180000
-
 high speed equilibrium sedimentation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.3.9 acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA sequencial random mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.9 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-malate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.9 trimer 3 * 66000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.9 additional information
-
 pI 7.5 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.9 0.019
-
 oxalate
-
 Saccharomyces cerevisiae
2.3.3.9 0.31
-
 glycolate
-
 Saccharomyces cerevisiae
2.3.3.9 0.54
-
 pyruvate
-
 Saccharomyces cerevisiae
2.3.3.9 1.5
-
 oxaloacetate
-
 Saccharomyces cerevisiae