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Literature summary extracted from

  • Hochstadt, J.
    Hypoxanthine phosphoribosyltransferase and guanine phosphoribosyltransferase from enteric bacteria (1978), Methods Enzymol., 51, 549-558.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.8 acetate slightly activating Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 acetate slightly activating Escherichia coli

General Stability

EC Number General Stability Organism
2.4.2.8 5-phospho-alpha-D-ribose 1-diphosphate stabilizes Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 5-phospho-alpha-D-ribose 1-diphosphate stabilizes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.8 6-aminopurine nucleotides
-
 Escherichia coli
2.4.2.8 6-aminopurine nucleotides
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 6-Hydroxypurine nucleotides
-
 Escherichia coli
2.4.2.8 6-Hydroxypurine nucleotides
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 Ba2+ strong Escherichia coli
2.4.2.8 Ba2+ strong Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 Ca2+ strong Escherichia coli
2.4.2.8 Ca2+ strong Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 nucleotides all free 5'-nucleotides are inhibitory, 6-OH purine nucleotides are most inhibitory, while 6-NH2 purine only at high concentrations Escherichia coli
2.4.2.8 nucleotides all free 5'-nucleotides are inhibitory, 6-OH purine nucleotides are most inhibitory, while 6-NH2 purine only at high concentrations Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 ppGpp strong Escherichia coli
2.4.2.8 ppGpp strong Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 Zn2+ strong Escherichia coli
2.4.2.8 Zn2+ strong Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.8 membrane
-
 Salmonella enterica subsp. enterica serovar Typhimurium 16020
-
2.4.2.8 membrane
-
 Escherichia coli 16020
-
2.4.2.8 periplasm
-
 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
2.4.2.8 periplasm
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+ absolute requirement for Mg2+ or Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 Mg2+ absolute requirement for Mg2+ or Mn2+ Escherichia coli
2.4.2.8 Mn2+ absolute requirement for Mg2+ or Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 Mn2+ absolute requirement for Mg2+ or Mn2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Escherichia coli
-
-
-
2.4.2.8 Escherichia coli
-
 phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes
-
2.4.2.8 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strain LT-2
-
2.4.2.8 Salmonella enterica subsp. enterica serovar Typhimurium
-
 phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.4.2.8 -20°C, dilute aqueous purified enzyme preparation, a few weeks Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 -20°C, dilute aqueous purified enzyme preparation, a few weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes, and catalyse the 2 reactions with different specificities Salmonella enterica subsp. enterica serovar Typhimurium GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes, and catalyse the 2 reactions with different specificities Escherichia coli GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes, and catalyse the 2 reactions with different specificities Salmonella enterica subsp. enterica serovar Typhimurium IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes, and catalyse the 2 reactions with different specificities Escherichia coli IMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium XMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli XMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 GPRT phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 GPRT phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes Escherichia coli
2.4.2.8 HPRT phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 HPRT phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 7.9
-
 guanine phosphoribosyltransferase Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 7.9
-
 guanine phosphoribosyltransferase Escherichia coli
2.4.2.8 8.4
-
 hypoxanthine phosphoribosyltransferase Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 8.4
-
 hypoxanthine phosphoribosyltransferase Escherichia coli
2.4.2.8 8.6
-
 substrate xanthine Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.8 8.6
-
 substrate xanthine Escherichia coli