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Literature summary extracted from

  • Dai, Y.P.; Lee, C.S.; O'Sullivan, W.J.
    Properties of uracil phosphoribosyltransferase from Giardia intestinalis (1995), Int. J. Parasitol., 25, 207-214.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.9 dGTP highly activating, no effect on Km values Giardia intestinalis
2.4.2.9 GTP highly activating, no effect on Km values Giardia intestinalis

General Stability

EC Number General Stability Organism
2.4.2.9 1 mM DTT and 0.1 mM EDTA stabilize the purified enzyme Giardia intestinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.9 1,3-dimethyl-uracil
-
 Giardia intestinalis
2.4.2.9 5-acetoxyuracil
-
 Giardia intestinalis
2.4.2.9 5-Bromouracil
-
 Giardia intestinalis
2.4.2.9 5-fluoro-deoxyuracil
-
 Giardia intestinalis
2.4.2.9 5-n-propyl-2'-deoxyuridine
-
 Giardia intestinalis
2.4.2.9 5-Nitrouracil
-
 Giardia intestinalis
2.4.2.9 6-benzyl-2-thiouracil
-
 Giardia intestinalis
2.4.2.9 6-formyluracil
-
 Giardia intestinalis
2.4.2.9 diphosphate noncompetitive against uracil, competitive against 5-phosphoribose 1-diphosphate Giardia intestinalis
2.4.2.9 DTNB 4 mM, 87% inhibition after 30 min Giardia intestinalis
2.4.2.9 tetrahydrouridine
-
 Giardia intestinalis
2.4.2.9 UMP competitive against uracil, noncompetitive against Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.9 0.024
-
 Uracil
-
 Giardia intestinalis
2.4.2.9 0.2
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.9 Ca2+ activates Giardia intestinalis
2.4.2.9 Cd2+ activates Giardia intestinalis
2.4.2.9 Co2+
-
 Giardia intestinalis
2.4.2.9 divalent cations preference of activation by divalent cations in decreasing order: Mn2+, Co2+, Cd2+, Mg2+, Ni2+, Ca2+, all 1 mM Giardia intestinalis
2.4.2.9 Mg2+
-
 Giardia intestinalis
2.4.2.9 Mn2+ required, 10 mM Giardia intestinalis
2.4.2.9 Mn2+ better activator than Mg2+ Giardia intestinalis
2.4.2.9 additional information no inhibition by any metal ion up to 10 mM Giardia intestinalis
2.4.2.9 Ni2+ activates Giardia intestinalis
2.4.2.9 Zn2+
-
 Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 36000
-
 2 * 36000, SDS-PAGE Giardia intestinalis
2.4.2.9 76000
-
 gel filtration Giardia intestinalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Giardia intestinalis
-
-
-
2.4.2.9 Giardia intestinalis Portland
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9
-
 Giardia intestinalis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.9 UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate rapid-random equilibrium mechanism Giardia intestinalis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.9 trophozoite
-
 Giardia intestinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.9 3.1
-
 purified enzyme Giardia intestinalis

Storage Stability

EC Number Storage Stability Organism
2.4.2.9 -20°C, 20 mM Hepes-KOH, pH 7.4, 1 mM DTT, stable for 3 days Giardia intestinalis
2.4.2.9 -70°C, 20 mM Hepes-KOH, pH 7.4, 1-2 months without loss of activity Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis Portland UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 dimer 2 * 36000, SDS-PAGE Giardia intestinalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.9 30
-
 assay at Giardia intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.9 6
-
-
 Giardia intestinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.9 5.5 8.5
-
 Giardia intestinalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.9 0.015
-
 UMP competitive versus uracil Giardia intestinalis
2.4.2.9 0.017
-
 tetrahydrouridine
-
 Giardia intestinalis
2.4.2.9 0.24
-
 diphosphate competitive versus 5-phosphoribose 1-diphosphate Giardia intestinalis