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Literature summary extracted from

  • Schwientek, T.; Yeh, J.C.; Levery, S.B.; Keck, B.; Merkx, G.; Van Kessel, A.G.; Fukuda, M.; Clausen, H.
    Control of O-glycan branch formation: molecular cloning and characterization of a novel thymus-associated core 2 beta1,6-N-acetylglucosaminyltransferase (2000), J. Biol. Chem., 275, 11106-11113.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.102 cloning and expression of isozyme 3 in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, secretion of the enzyme Homo sapiens
2.4.1.102 coexpression of isozyme 3 with leukosialin as substrate in CHO cells for in vivo activity assay of the enzyme Homo sapiens
2.4.1.102 DNA sequence determination and analysis Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.102 extracellular secretion into medium of the recombinant enzyme from Sf9 cells Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.102 additional information
-
 amino acid sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.102 Homo sapiens O95395 isozyme 2
-
2.4.1.102 Homo sapiens Q02742 isozyme 1
-
2.4.1.102 Homo sapiens Q9P109 isozyme 3
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.102 colon low content Homo sapiens
-
2.4.1.102 leukocyte peripheral blood Homo sapiens
-
2.4.1.102 ovary low content Homo sapiens
-
2.4.1.102 peripheral blood
-
 Homo sapiens
-
2.4.1.102 small intestine
-
 Homo sapiens
-
2.4.1.102 spleen low content Homo sapiens
-
2.4.1.102 testis low content Homo sapiens
-
2.4.1.102 thymus high content Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.102 additional information no activity human transferrin and IgG, N-acetylgalactosamine, core 3 substrates Homo sapiens ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + asialo-alpha1 acid glycoprotein no activity Homo sapiens ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + asialo-submaxillary mucin low activity Homo sapiens ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + asialo-submaxillary mucin bovine asialo-submaxillary mucin Homo sapiens ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + asialofetuin
-
 Homo sapiens ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + asialoglycophorin A best substrate Homo sapiens ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-methylumbelliferyl
-
 Homo sapiens UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-methylumbelliferyl
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-p-nitrophenyl
-
 Homo sapiens UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R substrate specificity Homo sapiens UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R i.e. core class 2 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R i.e. core class 1, R: polypeptide Homo sapiens UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R i.e. core class 2 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + fetuin no activity Homo sapiens ?
-
 ?