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Literature summary extracted from

  • Peterbauer, T.; Mucha, J.; Mach, L.; Richter, A.
    Chain Elongation of raffinose in pea seeds. Isolation, characterization, and molecular cloning of mutifunctional enzyme catalyzing the synthesis of stachyose and verbascose (2002), J. Biol. Chem., 277, 194-200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.67 cloned from maturing seeds, DNA sequence determination and analysis, functional expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.67 95000
-
 1 * 95000, about, SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.67 Pisum sativum Q93XK2 cv. Wunder von Kelvedon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.67
-
 Pisum sativum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.67 alpha-D-galactosyl-(1->3)-1D-myo-inositol + raffinose = myo-inositol + stachyose minimum double displacement mechanism Pisum sativum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.67 seed developing Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.67 0.5
-
 purified enzyme Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-ononitol 144.6% activity compared to raffinose Pisum sativum myo-inositol + alpha-D-galactosyl-D-ononitol
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-ononitol ononitol is 1-D-4-O-methyl-myo-inositol Pisum sativum myo-inositol + alpha-D-galactosyl-D-ononitol
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-pinitol 11.2% activity compared to raffinose Pisum sativum myo-inositol + galactopinitol A i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-pinitol D-pinitol is 1-D-3-O-methyl-chiro-inositol Pisum sativum myo-inositol + galactopinitol A i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + H2O hydrolysis Pisum sativum myo-inositol + D-galactose
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + raffinose i.e. galactinol Pisum sativum myo-inositol + stachyose
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + sequoyitol sequoyitol is 5-O-methyl-myo-inositol Pisum sativum myo-inositol + alpha-D-galactosyl-sequoyitol
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + sequoyitol 156.6% activity compared to raffinose Pisum sativum myo-inositol + alpha-D-galactosyl-sequoyitol
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + stachyose 12.1% activity compared to raffinose Pisum sativum myo-inositol + verbascose
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + verbascose
-
 Pisum sativum myo-inositol + ajugose
-
 ?
2.4.1.67 alpha-D-galactosyl-D-ononitol + raffinose 65.8% activity compared to 1-alpha-D-galactosyl-myo-inositol Pisum sativum D-ononitol + stachyose
-
 ?
2.4.1.67 alpha-D-galactosyl-D-ononitol + stachyose 9.9% activity compared to 1-alpha-D-galactosyl-myo-inositol Pisum sativum D-ononitol + verbascose
-
 ?
2.4.1.67 additional information native and recombinant enzyme show similar activity Pisum sativum ?
-
 ?
2.4.1.67 additional information sucrose is no acceptor substrate Pisum sativum ?
-
 ?
2.4.1.67 stachyose + stachyose
-
 Pisum sativum raffinose + verbascose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.67 monomer 1 * 95000, about, SDS-PAGE Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.67 stachyose synthase
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.67 30
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.67 7
-
-
 Pisum sativum