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Literature summary extracted from

  • Hoch, G.; Peterbauer, T.; Richter, A.
    Purification and characterization of stachyose synthase from lentil (Lens culinaris) seeds: galactopinitol and stachyose synthesis (1999), Arch. Biochem. Biophys., 366, 75-81.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.67 3.3
-
 1-alpha-D-galactosyl-myo-inositol with D-pinitol Lens culinaris
2.4.1.67 5.3
-
 1-alpha-D-galactosyl-myo-inositol with raffinose Lens culinaris
2.4.1.67 9.7
-
 raffinose with 1-alpha-D-galactosyl-myo-inositol Lens culinaris
2.4.1.67 12.4
-
 D-pinitol
-
 Lens culinaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.67 88600
-
 1 * 88600, SDS-PAGE Lens culinaris
2.4.1.67 99000
-
 gel filtration Lens culinaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + raffinose Lens culinaris
-
 myo-inositol + stachyose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.67 Lens culinaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.67
-
 Lens culinaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.67 seed mature Lens culinaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.67 additional information
-
 reaction kinetics Lens culinaris
2.4.1.67 0.55
-
 purified enzyme Lens culinaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-ononitol ononitol is 1-D-4-O-methyl-myo-inositol Lens culinaris myo-inositol + alpha-D-galactosyl-D-ononitol
-
 r
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-pinitol D-pinitol is 1-D-3-O-methyl-chiro-inositol Lens culinaris myo-inositol + galactopinitol A i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol r
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + galactopinitol A
-
 Lens culinaris myo-inositol + ciceritol i.e. O-alpha-D-galactopyranosyl-(1,6)-O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + raffinose
-
 Lens culinaris myo-inositol + stachyose
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + raffinose i.e. galactinol Lens culinaris myo-inositol + stachyose
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + sequoyitol sequoyitol is 5-O-methyl-myo-inositol Lens culinaris myo-inositol + alpha-D-galactosyl-sequoyitol
-
 ?
2.4.1.67 additional information substrate specificity, overview Lens culinaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.67 monomer 1 * 88600, SDS-PAGE Lens culinaris

Synonyms

EC Number Synonyms Comment Organism
2.4.1.67 stachyose synthase
-
 Lens culinaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.67 additional information
-
 pI: 4.8 Lens culinaris
2.4.1.67 7
-
-
 Lens culinaris