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Literature summary extracted from

  • He, M.; Yang, Z.Y.; Nie, Y.F.; Wang, J.; Xu, P.
    A new type of class I bacterial 5-enopyruvylshikimate-3-phosphate synthase mutants with enhanced tolerance to glyphosate (2001), Biochim. Biophys. Acta, 1568, 1-6.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expression of wild-type and chimeras of Salmonella and Escherichia coli mutant enzymes in Escherichia coli Escherichia coli
2.5.1.19 expression of wild-type and chimeras of Salmonella typhimurium and Escherichia coli mutant enzymes in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 N-phosphonomethylglycine
-
 Escherichia coli
2.5.1.19 N-phosphonomethylglycine
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 22.5
-
 phosphoenolpyruvate recombinant enzyme, in crude extracts Escherichia coli
2.5.1.19 43.6
-
 phosphoenolpyruvate recombinant enzyme, in crude extracts Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Escherichia coli
-
-
-
2.5.1.19 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 0.0058
-
 recombinant enzyme, activity in crude extracts Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 0.0064
-
 recombinant enzyme, activity in crude extracts Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 r

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.54
-
 N-phosphonomethylglycine recombinant enzyme, in crude extracts Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 0.96
-
 N-phosphonomethylglycine recombinant enzyme, in crude extracts Escherichia coli