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Literature summary extracted from

  • Mousdale, D.M.; Coggins, J.R.
    3-phosphoshikimate 1-carboxyvinyltransferase from Pisum sativum (1987), Methods Enzymol., 142, 348-354.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.19 prolonged dialysis against 50 mM Tris-HCl buffer, pH 7.5, 0.1 mM DTT, 1 mM benzamidine hydrochloride, 5 mM EDTA, 1 mM PMSF, stable to Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 N-phosphonomethylglycine
-
 Lactuca sativa
2.5.1.19 N-phosphonomethylglycine noncompetitive vs. phosphate, competitive vs. phosphoenolpyruvate, uncompetitive vs. shikimate 3-phosphate Pisum sativum
2.5.1.19 N-phosphonomethylglycine
-
 Spinacia oleracea
2.5.1.19 N-phosphonomethylglycine
-
 Triticum aestivum
2.5.1.19 N-phosphonomethylglycine
-
 Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.19 chloroplast
-
 Pisum sativum 9507
-
2.5.1.19 chloroplast
-
 Zea mays 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Triticum aestivum part of shikimate pathway phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Spinacia oleracea part of shikimate pathway phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Pisum sativum part of shikimate pathway phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Zea mays part of shikimate pathway phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Lactuca sativa part of shikimate pathway phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Lactuca sativa
-
 lettuce
-
2.5.1.19 Pisum sativum
-
 pea, cv. Onward
-
2.5.1.19 Spinacia oleracea
-
 spinach
-
2.5.1.19 Triticum aestivum
-
 wheat, cv. Maris Dove
-
2.5.1.19 Zea mays
-
 maize
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, cellulose phosphate Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.19 leaf
-
 Spinacia oleracea
-
2.5.1.19 seedling shoot tissue Pisum sativum
-
2.5.1.19 seedling shoot tissue Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 6.12
-
 phosphate + 5-enolpyruvylshikimate 3-phosphate Pisum sativum
2.5.1.19 12
-
 phosphoenolpyruvate + 3-phosphoshikimate Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.5.1.19 -20°C, 50% glycerol, 10-15% loss of activity per month Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Triticum aestivum phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Spinacia oleracea phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Pisum sativum phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Zea mays phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Lactuca sativa phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate part of shikimate pathway Triticum aestivum phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate part of shikimate pathway Spinacia oleracea phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate part of shikimate pathway Pisum sativum phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate part of shikimate pathway Zea mays phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate part of shikimate pathway Lactuca sativa phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.19 additional information no known cofactor Triticum aestivum
2.5.1.19 additional information no known cofactor Spinacia oleracea
2.5.1.19 additional information no known cofactor Pisum sativum
2.5.1.19 additional information no known cofactor Zea mays
2.5.1.19 additional information no known cofactor Lactuca sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.06 0.08 N-phosphonomethylglycine vs. shikimate 3-phosphate Pisum sativum
2.5.1.19 0.07 0.1 N-phosphonomethylglycine vs. phosphate Pisum sativum
2.5.1.19 0.08 0.1 N-phosphonomethylglycine vs. enolpyruvate Pisum sativum