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Literature summary extracted from

  • Dixon, S.C.; Martin, R.C.; Mok, M.C.; Shaw, G.; Mok, D.W.S.
    Zeatin glycosylation enzymes in Phaseolus - isolation of O-glucosyltransferase from Phaseolus lunatus and comparison to O-xylosyltransferase from P. vulgaris (1989), Plant Physiol., 90, 1316-1321.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.203 0.028
-
 trans-zeatin
-
 Phaseolus lunatus
2.4.1.203 0.216
-
 UDP-glucose
-
 Phaseolus lunatus
2.4.1.203 2.7
-
 UDP-xylose
-
 Phaseolus lunatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.203 44000
-
 gel filtration Phaseolus lunatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.203 UDP-glucose + zeatin Phaseolus lunatus important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.203 no activity in Phaseolus vulgaris
-
-
-
2.4.1.203 Phaseolus lunatus
-
 cv. Kingston
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.203
-
 Phaseolus lunatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.203 embryo immature Phaseolus lunatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.203 0.57
-
 purified enzyme Phaseolus lunatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.203 UDP-galactose + trans-zeatin
-
 Phaseolus lunatus UDP + O-galactosylzeatin
-
 ?
2.4.1.203 UDP-glucose + trans-zeatin cis-zeatin, ribosylzeatin, and dihydrozeatin are no substrates Phaseolus lunatus UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?
2.4.1.203 UDP-glucose + trans-zeatin specific for trans-zeatin Phaseolus lunatus UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?
2.4.1.203 UDP-glucose + zeatin important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues Phaseolus lunatus UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?
2.4.1.203 UDP-xylose + trans-zeatin
-
 Phaseolus lunatus UDP + O-xylosylzeatin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.203 27
-
 assay at Phaseolus lunatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.203 8
-
-
 Phaseolus lunatus