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Literature summary extracted from

  • Vavasseur, F.; Yang, J.M.; Dole, K.; Paulsen, H.; Brockhausen, I.
    Synthesis of O-glycan core 3: characterization of UDP-GlcNAc:GalNAc-R beta3-N-acetyl-glucosaminyltransferase activity from colonic mucosal tissues and lack of the activity in human cancer cell lines (1995), Glycobiology, 5, 351-357.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.147 NP 40 activation Rattus norvegicus
2.4.1.147 Triton X-100 stimulation, 0.1% v/v, inhibits at concentrations above 0.5% v/v Rattus norvegicus

General Stability

EC Number General Stability Organism
2.4.1.147 enzyme is extremly unstable in solubilized form Homo sapiens
2.4.1.147 enzyme is extremly unstable in solubilized form Rattus norvegicus
2.4.1.147 repeated freezing and thawing results in total loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.147 deoxycholate total inactivation Rattus norvegicus
2.4.1.147 N-iodoacetamido-galactosamine-alpha-benzyl colon homogenates, 50% inhibition at 2 mM Homo sapiens
2.4.1.147 NaCl slight inhibition at 0.1 M Rattus norvegicus
2.4.1.147 PMSF slight inhibition at 1 mM Rattus norvegicus
2.4.1.147 Triton X-100 0.5% reduces activity by 60% Rattus norvegicus
2.4.1.147 Triton X-114 slight stimulation Rattus norvegicus
2.4.1.147 UDP 60% reduced activity at 10 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.147 membrane
-
 Homo sapiens 16020
-
2.4.1.147 membrane
-
 Rattus norvegicus 16020
-
2.4.1.147 microsome
-
 Homo sapiens
-
-
2.4.1.147 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.102 Homo sapiens
-
-
-
2.4.1.147 Homo sapiens
-
-
-
2.4.1.147 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.102 colon
-
 Homo sapiens
-
2.4.1.102 HT-29 cell colonic adenocarcinoma cell line Homo sapiens
-
2.4.1.102 intestinal cancer cell line NCI498, ileo-caecal carcinoma cell line Homo sapiens
-
2.4.1.102 KG-1 cell erythroleukemia cell line Homo sapiens
-
2.4.1.102 LS-180 cell colonic adenocarcinoma cell line Homo sapiens
-
2.4.1.102 lymphocytic leukemia cell YC2C2 lymphocytic leukemia cell line Homo sapiens
-
2.4.1.102 additional information not in breast cancer cell lines Homo sapiens
-
2.4.1.102 SW-1116 cell colonic grade II tumour cell line Homo sapiens
-
2.4.1.102 SW-403 cell colonic grade III tumour cell line Homo sapiens
-
2.4.1.102 SW-48 cell colonic large ulcerating grade IV tumour cell line, low activity Homo sapiens
-
2.4.1.147 colon
-
 Homo sapiens
-
2.4.1.147 colon
-
 Rattus norvegicus
-
2.4.1.147 additional information no activity in diverse cancer cell lines Homo sapiens
-
2.4.1.147 additional information tissue-specific expression Homo sapiens
-
2.4.1.147 additional information tissue-specific expression Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R
-
 Homo sapiens UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + 2-azido-2-deoxy-alpha-D-galactosyl-benzyl
-
 Homo sapiens UDP + 3-O-(N-acetyl-beta-D-glucosaminyl)-2-azido-2-deoxy-alpha-D-galactosyl-benzyl
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + 4-nitrophenyl N-acetyl-alpha-D-galactosaminide
-
 Homo sapiens UDP + 4-nitrophenyl N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminide
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + alpha-D-galactosaminyl-4-nitrophenyl
-
 Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-alpha-D-galactosaminyl-4-nitrophenyl
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-(CH2)2NHCO(CH2)3CO2CH3
-
 Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-(CH2)2NHCO(CH2)3CO2CH3
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
-
 Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl best substrate Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R substrate specificity is significantly influenced by the structure of the aglycon group Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R substrate specificity is significantly influenced by the structure of the aglycon group Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + ovine submaxillary gland mucin acceptor substrate is desialylated, degalactosylated, defucosylated Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-mucin of ovine submaxillary gland
-
 ?
2.4.1.147 UDP-N-acetyl-D-glucosamine + ovine submaxillary gland mucin acceptor substrate is desialylated, degalactosylated, defucosylated Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-mucin of ovine submaxillary gland
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.102 More core 2 beta6-GalNAc-transferase, EC 2.4.1.102, exists in 2 types, type L and type M, the latter shows an additional core 4 beta6-GalNAc transferase activity Homo sapiens
2.4.1.102 More cf. EC 2.4.1.146-148 Homo sapiens
2.4.1.147 core 3 beta3-GlcNAc-T
-
 Homo sapiens
2.4.1.147 core 3 beta3-GlcNAc-T
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.147 37
-
 assay at Homo sapiens
2.4.1.147 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.147 6.5 7 assay at Homo sapiens
2.4.1.147 6.5 7 assay at Rattus norvegicus