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Literature summary extracted from

  • Taniguchi, M.; Tsuchiya, M.; Shimoyama, M.
    Comparison of acceptor protein specificities on the formation of ADP-ribose.acceptor adducts by arginine-specific ADP-ribosyltransferase from rabbit skeletal muscle sarcoplasmic reticulum with those of the enzyme from chicken peripheral polymorphonuclear cells (1993), Biochim. Biophys. Acta, 1161, 265-271.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.31 DNA enhances activity Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.31 K2HPO4 IC50: 50 mM Gallus gallus
2.4.2.31 K2HPO4 IC50: 40 mM Oryctolagus cuniculus
2.4.2.31 NaCl IC50: 50 mM Gallus gallus
2.4.2.31 NaCl IC50: 100 mM Oryctolagus cuniculus
2.4.2.31 novobiocin IC50: 0.2 mM Gallus gallus
2.4.2.31 novobiocin IC50: 0.4 mM Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.31 0.2
-
 NAD+
-
 Gallus gallus
2.4.2.31 0.2
-
 NAD+
-
 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.31 sarcoplasmic reticulum
-
 Oryctolagus cuniculus 16529
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.31 27500
-
 x * 27500, SDS-PAGE Oryctolagus cuniculus
2.4.2.31 40000
-
 x * 40000, SDS-PAGE Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.31 Gallus gallus
-
-
-
2.4.2.31 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.31
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.31 granulocyte peripheral polymorphonuclear Gallus gallus
-
2.4.2.31 skeletal muscle
-
 Gallus gallus
-
2.4.2.31 skeletal muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.31 1.32
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.31 actin + NAD+ no activity with the enzyme from skeletal muscle sarcoplasmic reticulum Oryctolagus cuniculus nicotinamide + ?
-
 ?
2.4.2.31 actin + NAD+ alpha-actin and beta/gamma-actin Gallus gallus nicotinamide + ?
-
 ?
2.4.2.31 casein + NAD+
-
 Gallus gallus nicotinamide + ?
-
 ?
2.4.2.31 P33 + NAD+ preferential endogenous acceptor Gallus gallus nicotinamide + ?
-
 ?
2.4.2.31 P33 + NAD+ no activity with the enzyme from skeletal muscle sarcoplasmic reticulum Oryctolagus cuniculus nicotinamide + ?
-
 ?
2.4.2.31 polyarginine + NAD+
-
 Gallus gallus nicotinamide + ?
-
 ?
2.4.2.31 polyarginine + NAD+
-
 Oryctolagus cuniculus nicotinamide + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.31 ?
-
 Gallus gallus
2.4.2.31 ? x * 40000, SDS-PAGE Oryctolagus cuniculus
2.4.2.31 ? x * 27500, SDS-PAGE Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.31 9
-
-
 Gallus gallus
2.4.2.31 9
-
-
 Oryctolagus cuniculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.2.31 0.2
-
 IC50: 0.2 mM Gallus gallus novobiocin
2.4.2.31 0.4
-
 IC50: 0.4 mM Oryctolagus cuniculus novobiocin
2.4.2.31 40
-
 IC50: 40 mM Oryctolagus cuniculus K2HPO4
2.4.2.31 50
-
 IC50: 50 mM Gallus gallus NaCl
2.4.2.31 50
-
 IC50: 50 mM Gallus gallus K2HPO4
2.4.2.31 100
-
 IC50: 100 mM Oryctolagus cuniculus NaCl