Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Moss, J.; Vaughan, M.
    NAD: arginine mono-ADP-ribosyltransferases from animal cells (1984), Methods Enzymol., 106, 430-437.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.31 Br- activates NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
2.4.2.31 Cl- activates NAD:arginine ADP-ribosyltransferase A, NaCl is maximally effective at 250 mM Meleagris gallopavo
2.4.2.31 F- activates NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
2.4.2.31 histone increases activity in absence of salt Meleagris gallopavo
2.4.2.31 lysophosphatidylcholine increases activity in the order of declining effectiveness C18, C16, C14, C12, C10, C8, NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
2.4.2.31 PO43- activates NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
2.4.2.31 SCN- activates NAD:arginine ADP-ribosyltransferase A, NaSCN is maximally effective at 100 mM Meleagris gallopavo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.31 0.015
-
 NAD+ NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
2.4.2.31 0.036
-
 NAD+ NAD:arginine ADP-ribosyltransferase B Meleagris gallopavo
2.4.2.31 1.3
-
 Arginine methyl ester in presence of 250 mM NaCl, NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
2.4.2.31 3
-
 Arginine methyl ester NAD:arginine ADP-ribosyltransferase B Meleagris gallopavo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.31 25300
-
 NAD:arginine ADP-ribosyltransferase A, gel filtration Meleagris gallopavo
2.4.2.31 28000
-
 1 * 28000, NAD:arginine ADP-ribosyltransferase A, SDS-PAGE Meleagris gallopavo
2.4.2.31 32000
-
 x * 32000, NAD:arginine ADP-ribosyltransferase B, SDS-PAGE Meleagris gallopavo

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.31 Meleagris gallopavo
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.31 NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
2.4.2.31 NAD:arginine ADP-ribosyltransferase B Meleagris gallopavo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.31 erythrocyte
-
 Meleagris gallopavo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.31 53
-
 NAD:arginine ADP-ribosyltransferase B Meleagris gallopavo
2.4.2.31 353
-
 NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo

Storage Stability

EC Number Storage Stability Organism
2.4.2.31 0-4°C, 50 mM sodium phosphate, pH 7.1, 100 mM NaCl, NAD:arginine ADP-ribosyltransferase B, stable Meleagris gallopavo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.31 L-arginine methyl ester + NADP+
-
 Meleagris gallopavo nicotinamide + N2-(2'-phospho-ADP-D-ribosyl)-L-arginine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.31 ? x * 32000, NAD:arginine ADP-ribosyltransferase B, SDS-PAGE Meleagris gallopavo
2.4.2.31 monomer 1 * 28000, NAD:arginine ADP-ribosyltransferase A, SDS-PAGE Meleagris gallopavo

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.2.31 NAD+ NAD+ is preferred over NADP+, NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
2.4.2.31 NADP+ NAD+ is preferred over NADP+, NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo