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Literature summary extracted from

  • Böttcher, F.; Adolph, R.D.; Hartmann, T.
    Homospermidine synthase, the first pathway-specific enzyme in pyrrolizidine alkaloid biosynthesis (1993), Phytochemistry, 32, 679-689.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.45 increased instability with higher purification grade Eupatorium cannabinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.45 1,3-diaminopropane competitive Eupatorium cannabinum
2.5.1.45 cadaverine mixed type inhibition Eupatorium cannabinum
2.5.1.45 Homospermidine competitive Eupatorium cannabinum
2.5.1.45 spermidine competitive Eupatorium cannabinum
2.5.1.45 spermine mixed type inhibition Eupatorium cannabinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.45 0.003
-
 NAD+ NAD+ functions as an enzyme-bound redoxsystem that is reduced in the first and reoxidized in the second part of the overall reaction. It functions as a coenzyme and not a cosubstrate Eupatorium cannabinum
2.5.1.45 0.0135
-
 putrescine in the absence of spermidine Eupatorium cannabinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.45 putrescine + spermine Eupatorium cannabinum
-
 sym-homospermine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermine Eupatorium pauciflorum
-
 sym-homospermine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermine Jacobaea vulgaris
-
 sym-homospermine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.45 Eupatorium cannabinum
-
-
-
2.5.1.45 Eupatorium pauciflorum
-
-
-
2.5.1.45 Jacobaea vulgaris
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.45 root
-
 Eupatorium cannabinum
-
2.5.1.45 root
-
 Eupatorium pauciflorum
-
2.5.1.45 root
-
 Jacobaea vulgaris
-
2.5.1.45 tissue culture
-
 Eupatorium cannabinum
-
2.5.1.45 tissue culture
-
 Eupatorium pauciflorum
-
2.5.1.45 tissue culture
-
 Jacobaea vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.45 0.047
-
-
 Eupatorium cannabinum

Storage Stability

EC Number Storage Stability Organism
2.5.1.45 4°C, crude extract, 0.5-1 mM spermidine, 0.5-1 mM NAD+, 1 week, 30% loss of activity Eupatorium cannabinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.45 putrescine + spermidine
-
 Eupatorium cannabinum N-(4-aminobutyl)butane-1,4-diamine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine
-
 Eupatorium pauciflorum N-(4-aminobutyl)butane-1,4-diamine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine
-
 Jacobaea vulgaris N-(4-aminobutyl)butane-1,4-diamine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermine
-
 Eupatorium cannabinum sym-homospermine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermine
-
 Eupatorium pauciflorum sym-homospermine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermine
-
 Jacobaea vulgaris sym-homospermine + propane-1,3-diamine
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.45 NAD+ the NADH formed in the first part of the reaction, i.e. oxidative deamination of putrescine, is cosubstrate of the second part of the reaction, i.e. hydrogenation of an intermolecular Schiff's base between the semialdehyde in the reaction and the second putrescine unit Eupatorium cannabinum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.45 0.0022
-
 spermine
-
 Eupatorium cannabinum
2.5.1.45 0.0063
-
 1,3-diaminopropane
-
 Eupatorium cannabinum
2.5.1.45 0.058
-
 cadaverine
-
 Eupatorium cannabinum
2.5.1.45 0.094
-
 spermidine
-
 Eupatorium cannabinum
2.5.1.45 0.95
-
 Homospermidine
-
 Eupatorium cannabinum