Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Abid, M.R.; Sasaki, K.; Titani, K.; Miyazaki, M.
    Biochemical and immunological characterization of deoxyhypusine synthase purified from the yeast Saccharomyces carlsbergensis (1997), J. Biochem., 121, 769-778.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.46 1,3-diaminopropane
-
 Saccharomyces cerevisiae
2.5.1.46 1,7-Diaminoheptane
-
 Saccharomyces cerevisiae
2.5.1.46 1,8-diaminooctane
-
 Saccharomyces cerevisiae
2.5.1.46 1,9-diaminononane
-
 Saccharomyces cerevisiae
2.5.1.46 Cu2+
-
 Saccharomyces cerevisiae
2.5.1.46 FAD
-
 Saccharomyces cerevisiae
2.5.1.46 Fe2+
-
 Saccharomyces cerevisiae
2.5.1.46 Fe3+
-
 Saccharomyces cerevisiae
2.5.1.46 FMN
-
 Saccharomyces cerevisiae
2.5.1.46 Mn2+ weak Saccharomyces cerevisiae
2.5.1.46 NADH
-
 Saccharomyces cerevisiae
2.5.1.46 spermidine
-
 Saccharomyces cerevisiae
2.5.1.46 Zn2+ weak Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.46 43000
-
 4 * 43000, SDS-PAGE Saccharomyces cerevisiae
2.5.1.46 165000 172000 gel filtration sedimentation equilibrium centrifugation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Saccharomyces cerevisiae [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine ?

Subunits

EC Number Subunits Comment Organism
2.5.1.46 tetramer 4 * 43000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.46 30
-
-
 Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.46 20 37 20°C: about 75% of maximal activity, 37°C: about 25% of maximal activity Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.46 4
-
 1 h, 35% loss of activity, NAD+ protects Saccharomyces cerevisiae
2.5.1.46 65
-
 15 min, almost complete loss of activity, NAD+ protects Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.46 9.5
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.46 8.5 11 pH 8.5: about 45% of maximal activity, pH 11.0: about 50% of maximal activity Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.46 NAD+
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.46 0.0009
-
 1,7-Diaminoheptane
-
 Saccharomyces cerevisiae
2.5.1.46 0.00191
-
 1,8-diaminooctane
-
 Saccharomyces cerevisiae
2.5.1.46 0.00649
-
 spermidine
-
 Saccharomyces cerevisiae
2.5.1.46 0.00752
-
 1,9-diaminononane
-
 Saccharomyces cerevisiae
2.5.1.46 0.0129
-
 1,3-diaminopropane
-
 Saccharomyces cerevisiae
2.5.1.46 0.85
-
 NADH
-
 Saccharomyces cerevisiae
2.5.1.46 1.28
-
 FMN
-
 Saccharomyces cerevisiae
2.5.1.46 2.27
-
 FAD
-
 Saccharomyces cerevisiae