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Literature summary extracted from

  • Lee, C.H.; Um, P.Y.; Park, M.H.
    Structure-function studies of human deoxyhypusine synthase: identification of amino acid residues critical for the binding of spermidine and NAD (2001), Biochem. J., 355, 841-849.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.46 D238A NAD-site mutant, less than 5% activity of wild-type Homo sapiens
2.5.1.46 D243A spermidine-site mutant, no spermidine-binding Homo sapiens
2.5.1.46 D313A unable to bind eIF5A-precursor, no synthesis of deoxyhypusine Homo sapiens
2.5.1.46 D316A spermidine-site mutant, no spermidine-binding Homo sapiens
2.5.1.46 D342A NAD-site mutant, less than 5% activity of wild-type Homo sapiens
2.5.1.46 E137A NAD-site mutant, less than 5% activity of wild-type Homo sapiens
2.5.1.46 E323A spermidine-site mutant, no spermidine-binding Homo sapiens
2.5.1.46 G283A NAD-site mutant, activity similar to wild-type Homo sapiens
2.5.1.46 H288A spermidine-site mutant, no spermidine-binding Homo sapiens
2.5.1.46 K305A spermidine-site mutant with retained avtivity Homo sapiens
2.5.1.46 K329A spermidine-site mutant, 18-24% spermidine binding capacity Homo sapiens
2.5.1.46 S317A NAD-site mutant, activity similar to wild-type Homo sapiens
2.5.1.46 T308A NAD-site mutant, activity similar to wild-type Homo sapiens
2.5.1.46 W327A spermidine-site mutant, no spermidine-binding Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.46
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.46 NAD+ one enzyme tetramer can bind up to four each of NAD+ and spermidine, the binding of spermidine being dependent on NAD+ Homo sapiens
2.5.1.46 NADH
-
 Homo sapiens