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Literature summary extracted from

  • Wolff, E.C.; Park, M.H.; Folk, J.E.
    Cleavage of spermidine as the first step in deoxyhypusine synthesis. The role of NAD+ (1990), J. Biol. Chem., 265, 4793-4799.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.46 0.00008
-
 [eIF5A-precursor]-L-lysine
-
 Rattus norvegicus
2.5.1.46 0.001
-
 spermidine
-
 Rattus norvegicus
2.5.1.46 0.03
-
 NAD+
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.46 cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.46 180000 190000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Rattus norvegicus
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.46
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.46 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.46 0.000137
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 spermidine reaction in absence of eIF5A-precursor Rattus norvegicus 1,3-diaminopropane + DELTA1-pyrroline
-
 ?
2.5.1.46 [eIF5A-precursor]-L-lysine + spermidine
-
 Rattus norvegicus [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Rattus norvegicus [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Rattus norvegicus [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.46 NAD+
-
 Rattus norvegicus
2.5.1.46 NADH
-
 Rattus norvegicus