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Literature summary extracted from

  • Becker, J.; Brendel, M.
    Rapid purification and characterization of two distinct N-deoxyribosyltransferases of Lactobacillus leichmannii (1996), Biol. Chem. Hoppe-Seyler, 377, 357-362.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.6 0.03
-
 guanine reactioin with deoxyinosine Lactobacillus leichmannii
2.4.2.6 0.04
-
 adenosine reaction with deoxyinosine Lactobacillus leichmannii
2.4.2.6 0.05
-
 thymidine reaction with cytosine Lactobacillus leichmannii
2.4.2.6 0.05
-
 cytosine reaction with thymidine Lactobacillus leichmannii
2.4.2.6 0.1
-
 deoxyadenosine reaction with cytosine Lactobacillus leichmannii
2.4.2.6 0.12
-
 cytosine reaction with deoxyadenosine Lactobacillus leichmannii
2.4.2.6 0.13
-
 thymine reaction with deoxyinosine Lactobacillus leichmannii
2.4.2.6 0.16
-
 deoxyguanosine reaction with cytosine Lactobacillus leichmannii
2.4.2.6 0.18
-
 cytosine reaction with deoxyinosine Lactobacillus leichmannii
2.4.2.6 0.19
-
 cytosine reaction with deoxyguanosine Lactobacillus leichmannii
2.4.2.6 0.27
-
 deoxyinosine reaction with guanine Lactobacillus leichmannii
2.4.2.6 0.3
-
 adenine reaction with deoxycytidine Lactobacillus leichmannii
2.4.2.6 0.3
-
 deoxyinosine reaction with thymine Lactobacillus leichmannii
2.4.2.6 0.33
-
 deoxyinosine reaction with adenosine Lactobacillus leichmannii
2.4.2.6 0.35
-
 deoxyinosine reaction with cytosine Lactobacillus leichmannii
2.4.2.6 0.48
-
 guanine reaction with deoxycytidine Lactobacillus leichmannii
2.4.2.6 0.48
-
 adenine reaction with thymidine Lactobacillus leichmannii
2.4.2.6 0.51
-
 thymidine reaction with adenine Lactobacillus leichmannii
2.4.2.6 0.52
-
 deoxycytidine reaction with guanine Lactobacillus leichmannii
2.4.2.6 0.52
-
 guanine reaction with thymidine Lactobacillus leichmannii
2.4.2.6 0.55
-
 deoxycytidine reaction with adenine Lactobacillus leichmannii
2.4.2.6 0.63
-
 thymidine reaction with guanine Lactobacillus leichmannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.6 18000
-
 6 * 18000, DRTase II, SDS-PAGE Lactobacillus leichmannii
2.4.2.6 20000
-
 6 * 20000, DRTAse I Lactobacillus leichmannii
2.4.2.6 110000
-
 DRTase II, non-denaturing PAGE Lactobacillus leichmannii
2.4.2.6 120000
-
 DRTase I, nondenaturing PAGE Lactobacillus leichmannii

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.6 Lactobacillus leichmannii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.6 DRTase I and DRTAse II Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.6 36.8
-
 DRTase II, reaction with thymidine + adenine Lactobacillus leichmannii
2.4.2.6 42.9
-
 DRTase I, reaction with deoxyinosine and adenine Lactobacillus leichmannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.6 2'-deoxycytidine + 6-chloroguanine DRTase II Lactobacillus leichmannii cytosine + 2'-deoxy-6-chloroguanosine
-
 ?
2.4.2.6 deoxyadenosine + cytosine DRTase II Lactobacillus leichmannii adenine + deoxycytidine
-
 ?
2.4.2.6 deoxycytidine + 1,7-dimethylguanine DRTase II Lactobacillus leichmannii cytosine + deoxy-1,7-dimethylguanosine
-
 ?
2.4.2.6 deoxycytidine + adenine
-
 Lactobacillus leichmannii cytosine + deoxyadenosine
-
 r
2.4.2.6 deoxycytidine + guanine
-
 Lactobacillus leichmannii cytosine + deoxyguanosine
-
 r
2.4.2.6 deoxyguanosine + cytosine
-
 Lactobacillus leichmannii guanine + deoxycytidine
-
 ?
2.4.2.6 deoxyinosine + 1,7-dimethylguanine DRTase I Lactobacillus leichmannii hypoxanthine + deoxy-1,7-dimethylguanosine
-
 ?
2.4.2.6 deoxyinosine + 6-chloroguanine DRTase I Lactobacillus leichmannii hypoxanthine + deoxy-6-chloroguanosine
-
 ?
2.4.2.6 deoxyinosine + adenine DRTase I Lactobacillus leichmannii hypoxanthine + deoxyadenosine
-
 r
2.4.2.6 deoxyinosine + cytosine DRTase II Lactobacillus leichmannii hypoxanthine + deoxycytidine
-
 ?
2.4.2.6 deoxyinosine + guanine DRTase I Lactobacillus leichmannii hypoxanthine + deoxyguanosine
-
 ?
2.4.2.6 deoxyinosine + thymine DRTase II Lactobacillus leichmannii hypoxanthine + thymidine
-
 ?
2.4.2.6 additional information DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine Lactobacillus leichmannii ?
-
 ?
2.4.2.6 thymidine + adenine DRTase II Lactobacillus leichmannii thymine + 2'-deoxyadenosine
-
 ?
2.4.2.6 thymidine + cytosine
-
 Lactobacillus leichmannii thymine + 2'-deoxycytidine
-
 ?
2.4.2.6 thymidine + guanine DRTase II Lactobacillus leichmannii thymine + 2'-deoxyguanosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.6 hexamer 6 * 18000, DRTase II, SDS-PAGE Lactobacillus leichmannii
2.4.2.6 hexamer 6 * 20000, DRTAse I Lactobacillus leichmannii

Synonyms

EC Number Synonyms Comment Organism
2.4.2.6 DRTase I strictly specific for transfer between purine bases Lactobacillus leichmannii
2.4.2.6 DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine Lactobacillus leichmannii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.6 45
-
 DRTase I Lactobacillus leichmannii
2.4.2.6 55
-
 DRTase II Lactobacillus leichmannii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.6 additional information
-
 additional information
-
 Lactobacillus leichmannii