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Literature summary extracted from

  • Miller, R.L.; Adamczyk, D.L.; Fyfe, J.A.; Elion, G.B.
    Xanthine phosphoribosyltransferase from Streptococcus faecalis. Properties and specificity (1974), Arch. Biochem. Biophys., 165, 349-358.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.22 increase in potassium phosphate buffer concentration from 1-50 mM increases stability Enterococcus faecalis
2.4.2.22 stabilizing compounds are: 5-phospho-alpha-D-ribose 1-diphosphate, XMP, 6-oxo-substituted purine nucleotides Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.22 1-Methylxanthine
-
 Enterococcus faecalis
2.4.2.22 2,4-dihydroxy-5,6-tetramethylenepyrimidine
-
 Enterococcus faecalis
2.4.2.22 2,4-Dihydroxypteridine
-
 Enterococcus faecalis
2.4.2.22 2,4-dihydroxypyrido[2,3-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 2,4-dihydroxypyrido[3,2-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 2,4-dihydroxypyrido[3,4-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 2,4-dihydroxypyrrolo[2,3-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 2,6-dithioxanthine
-
 Enterococcus faecalis
2.4.2.22 2-hydroxy-6-methylthiopurine
-
 Enterococcus faecalis
2.4.2.22 2-Hydroxypurine
-
 Enterococcus faecalis
2.4.2.22 2-mercaptopurine
-
 Enterococcus faecalis
2.4.2.22 2-methylhypoxanthine
-
 Enterococcus faecalis
2.4.2.22 2-methylthio-6-hydroxypurine
-
 Enterococcus faecalis
2.4.2.22 2-Thioxanthine
-
 Enterococcus faecalis
2.4.2.22 3-Methylxanthine
-
 Enterococcus faecalis
2.4.2.22 4,6-dihydroxypyrazolo[3,4-d]-pyrimidine
-
 Enterococcus faecalis
2.4.2.22 4-aminoimidazole-5-carboxamide
-
 Enterococcus faecalis
2.4.2.22 4-hydroxypyrazolo[3,4-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 4-mercapto-6-hydroxypyrazolo[3,4-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 4-mercaptopyrazolo[3,4-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 5,7-dihydroxy(1,2,5)thiadiazolo[3,4-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 5,7-dihydroxy-v-triazolo[4,5-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 5,7-dihydroxypyrazolo[4,3-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 5-acetamidouracil
-
 Enterococcus faecalis
2.4.2.22 5-Aminouracil
-
 Enterococcus faecalis
2.4.2.22 5-anilinouracil
-
 Enterococcus faecalis
2.4.2.22 5-formamidouracil
-
 Enterococcus faecalis
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate
-
 Enterococcus faecalis
2.4.2.22 6-Mercaptopurine
-
 Enterococcus faecalis
2.4.2.22 6-Thioxanthine
-
 Enterococcus faecalis
2.4.2.22 7-methylxanthine
-
 Enterococcus faecalis
2.4.2.22 8-mercaptoxanthine
-
 Enterococcus faecalis
2.4.2.22 8-methylxanthine
-
 Enterococcus faecalis
2.4.2.22 9-methylxanthine
-
 Enterococcus faecalis
2.4.2.22 adenine
-
 Enterococcus faecalis
2.4.2.22 ATP 1 mM, 11% inhibition Enterococcus faecalis
2.4.2.22 CDP 1 mM, 10% inhibition Enterococcus faecalis
2.4.2.22 CTP 1 mM, 27% inhibition Enterococcus faecalis
2.4.2.22 GDP 1 mM, 93 inhibition Enterococcus faecalis
2.4.2.22 GMP 1 mM, 89% inhibition Enterococcus faecalis
2.4.2.22 GTP 1 mM, 91% inhibition Enterococcus faecalis
2.4.2.22 guanine
-
 Enterococcus faecalis
2.4.2.22 hypoxanthine
-
 Enterococcus faecalis
2.4.2.22 isoguanine
-
 Enterococcus faecalis
2.4.2.22 purine
-
 Enterococcus faecalis
2.4.2.22 TDP 1 mM, 18% inhibition Enterococcus faecalis
2.4.2.22 TTP 1 mM, 13% inhibition Enterococcus faecalis
2.4.2.22 UDP 1 mM, 15% inhibition Enterococcus faecalis
2.4.2.22 Uracil
-
 Enterococcus faecalis
2.4.2.22 uric acid
-
 Enterococcus faecalis
2.4.2.22 UTP 1 mM, 17% inhibition Enterococcus faecalis
2.4.2.22 xanthine
-
 Enterococcus faecalis
2.4.2.22 XDP 1 mM, 89% inhibition Enterococcus faecalis
2.4.2.22 XMP 1 mM, 92% inhibition Enterococcus faecalis
2.4.2.22 XTP 1 mM, 92% inhibition Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.22 0.019
-
 5,7-dihydroxy-v-triazolo[4,5-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 0.02
-
 xanthine
-
 Enterococcus faecalis
2.4.2.22 0.053
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.22 42000
-
 gel filtration Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.22 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.22
-
 Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.22 3.65
-
-
 Enterococcus faecalis

Storage Stability

EC Number Storage Stability Organism
2.4.2.22 -70°C, stable for 6 months in intacts cells Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + 4,6-dihydroxypyrazolo[3,4-d]pyrimidine at 0.064% of the activity with xanthine Enterococcus faecalis ?
-
 ?
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + 5,7-dihydroxy-v-triazolo[4,5-d]pyrimidine at 16.8% of the activity with xanthine Enterococcus faecalis ?
-
 ?
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + adenine at 0.25% of the activity with xanthine Enterococcus faecalis AMP + diphosphate
-
 ?
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine at 0.19% of the activity with xanthine Enterococcus faecalis IMP + diphosphate
-
 ?
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + xanthine
-
 Enterococcus faecalis 9-(5-phospho-beta-D-ribosyl)xanthine + diphosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.22 25
-
 pH 5.6-10, stable for 30 min Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.22 7.4 8.8
-
 Enterococcus faecalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.22 6.9 9.1 80% of maximal activity at pH 6.9 and 9.1 Enterococcus faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.22 5.6 10 stable Enterococcus faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.22 0.0022
-
 5,7-dihydroxypyrazolo[4,3-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 0.0041
-
 2,4-dihydroxypyrido[3,2-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 0.021
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Enterococcus faecalis
2.4.2.22 0.024
-
 xanthine
-
 Enterococcus faecalis
2.4.2.22 0.024
-
 2,4-Dihydroxypteridine
-
 Enterococcus faecalis
2.4.2.22 0.032
-
 5,7-dihydroxy(1,2,5)thiadiazolo[3,4-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 0.11
-
 5,7-dihydroxy-v-triazolo[4,5-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 0.15
-
 2,6-dithioxanthine
-
 Enterococcus faecalis
2.4.2.22 0.17
-
 2-hydroxy-6-methylthiopurine
-
 Enterococcus faecalis
2.4.2.22 0.2
-
 4-mercapto-6-hydroxypyrazolo[3,4-d]pyrimidine
-
 Enterococcus faecalis
2.4.2.22 0.31
-
 4,6-dihydroxypyrazolo[3,4-d]-pyrimidine
-
 Enterococcus faecalis
2.4.2.22 0.34
-
 6-Thioxanthine
-
 Enterococcus faecalis
2.4.2.22 0.38
-
 2-Thioxanthine
-
 Enterococcus faecalis
2.4.2.22 0.39
-
 8-mercaptoxanthine
-
 Enterococcus faecalis
2.4.2.22 0.62
-
 3-Methylxanthine
-
 Enterococcus faecalis