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Literature summary extracted from

  • Ushiro, H.; Yokoyama, Y.; Shizuta, Y.
    Purification and characterization of poly (ADP-ribose) synthetase from human placenta (1987), J. Biol. Chem., 262, 2352-2357.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.30 DNA absolute requirement Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.30 3-aminobenzamide 1 mM, 99% inhibition Homo sapiens
2.4.2.30 3-aminobenzoic acid 1 mM, 12% inhibition Homo sapiens
2.4.2.30 Cu2+
-
Homo sapiens
2.4.2.30 Hg2+
-
Homo sapiens
2.4.2.30 nicotinamide 1 mM, 93% inhibition Homo sapiens
2.4.2.30 PCMB complete Homo sapiens
2.4.2.30 Zn2+
-
Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.30 Ba2+ enhances activity Homo sapiens
2.4.2.30 Mg2+ enhances both the automodification and poly(ADP-ribosyl)ation of histone H1 Homo sapiens
2.4.2.30 Sr2+ enhances activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.30 116000
-
1 * 116000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.30 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.30
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.30 placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.30 1.02
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor poly(ADP-ribosyl)ation of histone H1 Homo sapiens nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
?
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor catalyzes poly(ADP-ribosyl)ation of the synthetase itself, automodification Homo sapiens nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.30 monomer 1 * 116000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.30 8.5
-
reaction with histone in presence of DNA, 0.1 M glycine-NaOH buffer Homo sapiens
2.4.2.30 8.7
-
reaction with histone in presence of DNA, 0.1 M Tris-HCl buffer Homo sapiens