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Literature summary extracted from

  • Mozer, T.J.; Tiemeier, D.C.; Jaworski, E.G.
    Purification and characterization of corn glutathione S-transferase (1983), Biochemistry, 22, 1068-1072.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.6
-
 alachlor i.e. 2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide Zea mays
2.5.1.18 2.8
-
 1-chloro-2,4-dinitrobenzene GST II Zea mays
2.5.1.18 3
-
 1-chloro-2,4-dinitrobenzene GST I Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.18 K+ no enzyme activity with potassium phosphate concentrations below 20 mM, optimal concentration 50 mM Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 29000
-
 2 * 29000, enzyme GST II, SDS-PAGE Zea mays
2.5.1.18 29000
-
 2 * 29000, enzyme GST I, SDS-PAGE Zea mays
2.5.1.18 50000
-
 gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Zea mays
-
 2 forms: GST I, GST II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 2 forms: GST I, GST II Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 leaf etiolated Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 21
-
 GST II Zea mays
2.5.1.18 66
-
 GST I Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + alachlor i.e. 2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide Zea mays ?
-
 ?
2.5.1.18 RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Zea mays HX + R-S-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 29000, enzyme GST II, SDS-PAGE Zea mays
2.5.1.18 dimer 2 * 29000, enzyme GST I, SDS-PAGE Zea mays