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Literature summary extracted from

  • Asaoka, K.
    Affinity purification and characterization of glutathione S-transferases from bovine liver (1984), J. Biochem., 95, 685-696.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.18 glycerol, 30%, protects against thermal inactivation Bos taurus
2.5.1.18 sucrose, 0.5 M, protects against thermal inactivation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 butanol 50% inactivation at 5% Bos taurus
2.5.1.18 Cd2+
-
 Bos taurus
2.5.1.18 Co2+ weak Bos taurus
2.5.1.18 Cu2+
-
 Bos taurus
2.5.1.18 Hg2+
-
 Bos taurus
2.5.1.18 Ni2+ weak Bos taurus
2.5.1.18 Zn2+
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.1
-
 glutathione isozyme I Bos taurus
2.5.1.18 0.16
-
 glutathione isozyme II Bos taurus
2.5.1.18 0.55
-
 1-chloro-2,4-dinitrobenzene isozyme I Bos taurus
2.5.1.18 1.3
-
 3,4-dinitrobenzoic acid isozymes I and II Bos taurus
2.5.1.18 2
-
 1,2-dinitrobenzene isozymes I and II Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 27000
-
 2 * 27000, SDS-PAGE Bos taurus
2.5.1.18 49000
-
 gel filtration Bos taurus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.5.1.18 Acetone 25% acetone: 70-80% inactivation Bos taurus
2.5.1.18 Acetone 5% acetone: stable Bos taurus
2.5.1.18 Butanol stable at 5% butanol Bos taurus
2.5.1.18 dimethyl sulfoxide stable at 5% and 25% dimethyl sulfoxide Bos taurus
2.5.1.18 dimethylformamide 5% dimethylformamide: stable, 25% dimethylformamide: 70-80% inactivation Bos taurus
2.5.1.18 dioxane 5% dioxane: stable Bos taurus
2.5.1.18 dioxane 25% dioxane: 70-80% inactivation Bos taurus
2.5.1.18 Ethanol 25% ethanol: 70-80% inactivation Bos taurus
2.5.1.18 Ethanol 5% ethanol: stable Bos taurus
2.5.1.18 Methanol 25% methanol: 70-80% inactivation Bos taurus
2.5.1.18 Methanol 5% methanol: stable Bos taurus
2.5.1.18 methyl cellosolve stable at 5% and 25% methyl cellosolve for 2 h Bos taurus
2.5.1.18 propanol 25% propanol: 70-80% inactivation Bos taurus
2.5.1.18 propanol 5% propanol: stable Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Bos taurus
-
 liver: 2 major forms: I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 2.3
-
 with 1,2-dinitrobenzene Bos taurus
2.5.1.18 15
-
 with 1-chloro-2,4-dinitrobenzene Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.5.1.18 4°C, 50% loss of activity after 18 h Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dinitrobenzene
-
 Bos taurus ?
-
 ?
2.5.1.18 glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Bos taurus ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Bos taurus S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 3,4-dinitrobenzoic acid
-
 Bos taurus ?
-
 ?
2.5.1.18 glutathione + 5-nitrofurfural
-
 Bos taurus ?
-
 ?
2.5.1.18 glutathione + 5-nitrofurfural diacetal
-
 Bos taurus ?
-
 ?
2.5.1.18 additional information specificity Bos taurus ?
-
 ?
2.5.1.18 RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Bos taurus HX + R-S-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 27000, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 30
-
 3 h, stable Bos taurus
2.5.1.18 35
-
 1 h, stable Bos taurus
2.5.1.18 37
-
 fairly stable between pH 6 and 10 Bos taurus
2.5.1.18 40
-
 10 min, stable Bos taurus
2.5.1.18 55
-
 above, 10 min, 50% inactivation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 6 8 pH 6: about 40% of activity maximum, pH 8: about 55% of activity maximum Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.18 3.5
-
 10 min, 37°C, 50% loss of activity, 18 h at 4°C, 50% loss of activity Bos taurus
2.5.1.18 6 10 10 min at 37°C, stable, 18 h at 4°C, stable Bos taurus