Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rajashekhara, E.; Kitaoka, M.; Kim, Y.K.; Hayashi, K.
    Characterization of a cellobiose phosphorylase from a hyperthermophilic eubacterium, Thermotoga maritima MSB8 (2002), Biosci. Biotechnol. Biochem., 66, 2578-2586.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.20 expressed in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.20 D-glucose substrate inhibition, competitive with glucose-1-phosphate Thermotoga maritima
2.4.1.20 D-glucose 1-phosphate
-
 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.20 0.15
-
 phosphate
-
 Thermotoga maritima
2.4.1.20 0.29
-
 cellobiose
-
 Thermotoga maritima
2.4.1.20 0.69
-
 D-glucose
-
 Thermotoga maritima
2.4.1.20 4.1
-
 6-deoxy-D-glucose
-
 Thermotoga maritima
2.4.1.20 5.7
-
 D-glucosamine
-
 Thermotoga maritima
2.4.1.20 14
-
 D-xylose
-
 Thermotoga maritima
2.4.1.20 47
-
 2-deoxy-D-glucose
-
 Thermotoga maritima
2.4.1.20 67
-
 D-mannose
-
 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.20 85000
-
 SDS-PAGE Thermotoga maritima
2.4.1.20 94320
-
 calculated from DNA-sequence Thermotoga maritima
2.4.1.20 150000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.20 cellobiose + phosphate Thermotoga maritima
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Thermotoga maritima
-
-
-
2.4.1.20 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.20
-
 Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.20 2.66
-
 purified enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Thermotoga maritima ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
-
 Thermotoga maritima ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucosamine
-
 Thermotoga maritima ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-mannose
-
 Thermotoga maritima ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-xylose no activity with L-isomer Thermotoga maritima ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-xylose higher specific activity than with D-glucose as acceptor Thermotoga maritima ?
-
 ?
2.4.1.20 cellobiose + phosphate
-
 Thermotoga maritima alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate random ordered bi-bi mechanism Thermotoga maritima alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate sequential bi-bi mechanism Thermotoga maritima alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate random ordered bi-bi mechanism Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate sequential bi-bi mechanism Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.20 dimer 2 * 85000, gel filtration, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.20 80
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.20 40 95
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.20 70
-
 less than 10% loss of activity after heating for 30 min at pH 6-8 Thermotoga maritima
2.4.1.20 90
-
 25% loss of activity after heating for 30 min at pH 6-8 Thermotoga maritima
2.4.1.20 100
-
 complete loss of activity after heating for 30 min at pH 6-8 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.20 4.4
-
 D-mannose
-
 Thermotoga maritima
2.4.1.20 5.2
-
 D-glucosamine
-
 Thermotoga maritima
2.4.1.20 5.4
-
 cellobiose
-
 Thermotoga maritima
2.4.1.20 8
-
 D-glucose
-
 Thermotoga maritima
2.4.1.20 16
-
 2-deoxy-D-glucose
-
 Thermotoga maritima
2.4.1.20 17
-
 6-deoxy-D-glucose
-
 Thermotoga maritima
2.4.1.20 40
-
 D-xylose
-
 Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.20 6.2
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.20 4 9
-
 Thermotoga maritima