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Literature summary extracted from

  • Silverstein, R.; Voet, J.; Reed, D.; Abeles, R.H.
    Purification and mechanism of action of sucrose phosphorylase (1967), J. Biol. Chem., 242, 1338-1346.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.7 80000 100000 gel filtration Pelomonas saccharophila
2.4.1.7 84000
-
 sedimetation equilibrium centrifugation Pelomonas saccharophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.7 sucrose + phosphate Pelomonas saccharophila double displacement mechanism alpha-D-glucose 1-phosphate + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Pelomonas saccharophila
-
 ATCC 9114
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7 less than 10% impurities Pelomonas saccharophila

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.7 sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate double displacement mechanism Pelomonas saccharophila
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 alpha-D-glucose 1-phosphate + D-xylulose
-
 Pelomonas saccharophila alpha-D-glucopyranosyl-D-xylulofuranoside + phosphate
-
 r
2.4.1.7 alpha-D-glucose 1-phosphate + H2O
-
 Pelomonas saccharophila alpha-D-glucose + phosphate
-
 ir
2.4.1.7 alpha-D-glucose 1-phosphate + L-arabinose
-
 Pelomonas saccharophila ?
-
 r
2.4.1.7 alpha-D-glucose 1-phosphate + L-arabinulose
-
 Pelomonas saccharophila ?
-
 r
2.4.1.7 alpha-D-glucose-1-phosphate + L-sorbose
-
 Pelomonas saccharophila alpha-D-glucosyl-alpha-L-sorbose + phosphate
-
 r
2.4.1.7 glucose-1-phosphate + arsenate
-
 Pelomonas saccharophila glucose-1-arsenate + phosphate glucose-1-arsenate is further hydrolyzed to form glucose and arsenate ir
2.4.1.7 additional information sucrose and alpha-glucose-1-phosphate are hydrolyzed in absence of phosphate and arsenate at very low rate Pelomonas saccharophila ?
-
 ?
2.4.1.7 sucrose + phosphate double displacement mechanism Pelomonas saccharophila alpha-D-glucose 1-phosphate + D-fructose
-
 ?
2.4.1.7 sucrose + phosphate double displacement mechanism Pelomonas saccharophila alpha-D-glucose 1-phosphate + D-fructose
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 6.3
-
 hydrolysis Pelomonas saccharophila
2.4.1.7 7
-
 phosphorylysis Pelomonas saccharophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.7 5.5 9
-
 Pelomonas saccharophila