Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shimizu, H.; Yamagata, S.; Masui, R.; Inoue,Y.; Shibata, T.; Yokoyama, S.; Kuramitsu, S.; Iwama, T.
    Cloning and overexpression of the oah1 gene encoding O-acetyl-L-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product (2001), Biochim. Biophys. Acta, 1549, 61-72.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.49 oah1 gene cloned, sequenced and overexpressed in Escherichia coli BL21(DE3) Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 DL-C-propagylglycine
-
Thermus thermophilus
2.5.1.49 Hydroxylamine hydrochloride
-
Thermus thermophilus
2.5.1.49 L-methionine
-
Thermus thermophilus
2.5.1.49 phenylhydrazine
-
Thermus thermophilus
2.5.1.49 Semicarbazide
-
Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 1.3
-
H2S
-
Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.49 46000
-
4 * 46000, calculated from OAH1 sequence Thermus thermophilus
2.5.1.49 46059
-
4 * 46059, calculated from amino acid sequence Thermus thermophilus
2.5.1.49 47000
-
4 * 47000, SDS-PAGE Thermus thermophilus
2.5.1.49 163000
-
gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Thermus thermophilus Q93I77 DDBJ: AB049221, EMBL:AB049221
-
2.5.1.49 Thermus thermophilus Q93I77 nucleotide sequence accession no.
-
2.5.1.49 Thermus thermophilus Q93I77 HB8, HB27
-
2.5.1.49 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q93I77 DDBJ: AB049221, EMBL:AB049221
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49 recombinant enzyme Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 0.06
-
cysthathione beta-lyase reaction Thermus thermophilus
2.5.1.49 0.58
-
L-homoserine as substrate Thermus thermophilus
2.5.1.49 0.72
-
O-succinyl-L-homoserine as substrate Thermus thermophilus
2.5.1.49 1.33
-
L-serine as substrate Thermus thermophilus
2.5.1.49 1.57
-
O-phospho-L-serine as substrate Thermus thermophilus
2.5.1.49 2.12
-
O-acetyl-L-serine as substrate Thermus thermophilus
2.5.1.49 40.7
-
recombinant enzyme Thermus thermophilus
2.5.1.49 49.82
-
O-acetyl-L-homoserine as substrate Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 L-homoserine + H2S
-
Thermus thermophilus homocysteine + ?
-
?
2.5.1.49 L-homoserine + H2S
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 homocysteine + ?
-
?
2.5.1.49 additional information low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction Thermus thermophilus ?
-
?
2.5.1.49 additional information low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
?
2.5.1.49 O-acetyl-L-serine + H2S
-
Thermus thermophilus L-cysteine + acetic acid
-
?
2.5.1.49 O-acetyl-L-serine + H2S
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-cysteine + acetic acid
-
?
2.5.1.49 O-phospho-L-serine + H2S
-
Thermus thermophilus ?
-
?
2.5.1.49 O-phospho-L-serine + H2S
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
?
2.5.1.49 O-succinyl-L-homoserine + H2S
-
Thermus thermophilus homocysteine + ?
-
?
2.5.1.49 O-succinyl-L-homoserine + H2S
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 homocysteine + ?
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.49 tetramer 4 * 47000, SDS-PAGE Thermus thermophilus
2.5.1.49 tetramer 4 * 46059, calculated from amino acid sequence Thermus thermophilus
2.5.1.49 tetramer 4 * 46000, calculated from OAH1 sequence Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.49 70
-
-
Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.49 60 85
-
Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.49 90
-
very stable at high temperatures, 90% activity at 90°C for 60 min at pH 7.8 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 7.8
-
-
Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.49 4 12 50% maximum activity at pH 6.3 and pH 9.3 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate
-
Thermus thermophilus