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Literature summary extracted from

  • Yamagata, S.; Isaji, M.; Nakamura, K.; Fujisaki, S.; Doi, K.; Bawden, S.; D'Andrea, R.
    Overexpression of the Saccharomyces cerevisiae MET17/MET25 gene in Escherichia coli and comparative characterization of the product with O-acetylserine-O-acetylhomoserine sulfhydrylase of the yeast (1994), Appl. Microbiol. Biotechnol., 42, 92-99.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.49 MET17/MET25 gene cloned and overexpressed in Escherichia coli Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.5.1.49 sensitive to trypsin digestion Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 Hydroxylamine hydrochloride
-
 Saccharomyces cerevisiae
2.5.1.49 L-Penicillamine
-
 Saccharomyces cerevisiae
2.5.1.49 phenylhydrazine
-
 Saccharomyces cerevisiae
2.5.1.49 Semicarbazide
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 8.7
-
 O-acetyl-L-homoserine wild-type enzyme Saccharomyces cerevisiae
2.5.1.49 9
-
 O-acetyl-L-homoserine recombinant enzyme Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49 wild-type and recombinant enzyme Saccharomyces cerevisiae