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Literature summary extracted from

  • Ozaki, H.; Shiio, I.
    Methionine biosynthesis in Brevibacterium flavum: Properties and essential role of O-acetylhomoserine sulfhydrylase (1982), J. Biochem., 91, 1163-1171.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.49 DL-cystathionine 10 mM addition, AHS activity 105% [Brevibacterium] flavum
2.5.1.49 pyridoxal 5'-phosphate
-
 [Brevibacterium] flavum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 L-homoserine
-
 [Brevibacterium] flavum
2.5.1.49 L-methionine
-
 [Brevibacterium] flavum
2.5.1.49 additional information L-serine, glycine, cystathione and S-adenosyl-L-methionine are no inhibitors [Brevibacterium] flavum
2.5.1.49 O-acetyl-L-serine
-
 [Brevibacterium] flavum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 0.083
-
 H2S
-
 [Brevibacterium] flavum
2.5.1.49 2
-
 O-acetyl-L-homoserine
-
 [Brevibacterium] flavum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.49 360000
-
 gel filtration [Brevibacterium] flavum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + H2S [Brevibacterium] flavum
-
 L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methanethiol [Brevibacterium] flavum methionine biosynthetic pathway L-methionine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 [Brevibacterium] flavum
-
 No. 2247, ATCC 14067
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49
-
 [Brevibacterium] flavum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 0.00472
-
-
 [Brevibacterium] flavum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 additional information shows with O-acetylserine 1% of the activity with O-acetylhomoserine, no activity with O-succinyl-L-homoserine, homoserine or serine [Brevibacterium] flavum ?
-
 ?
2.5.1.49 O-acetyl-L-homoserine + H2S
-
 [Brevibacterium] flavum L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methanethiol methionine biosynthetic pathway [Brevibacterium] flavum L-methionine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.49 AHS
-
 [Brevibacterium] flavum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 8.7
-
-
 [Brevibacterium] flavum

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate
-
 [Brevibacterium] flavum