Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Murooka,Y.; Kakihara, K.; Miwa, T.; Seto, K.; Harada, T.
    O-Alkylhomoserine synthesis catalyzed by O-acetylhomoserine sulfhydrylase in microorganisms (1977), J. Bacteriol., 130, 62-73.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.5.1.49 not stable even in presence of a cofactor Corynebacterium acetophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 butanol
-
 Corynebacterium acetophilum
2.5.1.49 hydrazine
-
 Corynebacterium acetophilum
2.5.1.49 Hydroxylamine hydrochloride
-
 Corynebacterium acetophilum
2.5.1.49 Isopropanol
-
 Corynebacterium acetophilum
2.5.1.49 KCN
-
 Corynebacterium acetophilum
2.5.1.49 L-homoserine
-
 Corynebacterium acetophilum
2.5.1.49 methanol
-
 Corynebacterium acetophilum
2.5.1.49 methylmercaptan
-
 Corynebacterium acetophilum
2.5.1.49 n-Propanol
-
 Corynebacterium acetophilum
2.5.1.49 Na2S
-
 Corynebacterium acetophilum
2.5.1.49 O-acetyl-L-serine
-
 Corynebacterium acetophilum
2.5.1.49 O-acetyl-L-threonine
-
 Corynebacterium acetophilum
2.5.1.49 O-ethylhomoserine
-
 Corynebacterium acetophilum
2.5.1.49 O-succinyl-DL-homoserine
-
 Corynebacterium acetophilum
2.5.1.49 p-chloromercuribenzoate
-
 Corynebacterium acetophilum
2.5.1.49 phenylhydrazine
-
 Corynebacterium acetophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 1.1
-
 O-acetyl-L-homoserine O-alkylhomoserine synthesis Corynebacterium acetophilum
2.5.1.49 2.4
-
 H2S
-
 Corynebacterium acetophilum
2.5.1.49 13
-
 O-acetyl-L-homoserine O-acetylhomoserine sulfhydrylation Corynebacterium acetophilum
2.5.1.49 40
-
 ethanol
-
 Corynebacterium acetophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.49 110000
-
 2 * 110000, SDS-PAGE Corynebacterium acetophilum
2.5.1.49 220000
-
 gel filtration Corynebacterium acetophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + H2S Corynebacterium acetophilum
-
 L-homocysteine + acetic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Corynebacterium acetophilum
-
-
-
2.5.1.49 Corynebacterium acetophilum A51
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49
-
 Corynebacterium acetophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 0.108
-
 O-alkylhomoserine synthesizing activity Corynebacterium acetophilum
2.5.1.49 16.7
-
 O-acetylhomoserine sulfhydrylase activity Corynebacterium acetophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 additional information O-acetyl-L-serine, L-homoserine, O-succinyl-L-homoserine and O-acetyl-L-threonine are no substrates Corynebacterium acetophilum ?
-
 ?
2.5.1.49 additional information O-acetyl-L-serine, L-homoserine, O-succinyl-L-homoserine and O-acetyl-L-threonine are no substrates Corynebacterium acetophilum A51 ?
-
 ?
2.5.1.49 O-acetyl-L-homoserine + ethanol
-
 Corynebacterium acetophilum O-ethyl-homoserine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + ethanol
-
 Corynebacterium acetophilum A51 O-ethyl-homoserine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + H2S
-
 Corynebacterium acetophilum L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methanol
-
 Corynebacterium acetophilum O-methyl-homoserine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methyl mercaptan
-
 Corynebacterium acetophilum L-methionine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methyl mercaptan
-
 Corynebacterium acetophilum A51 L-methionine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + n-butanol
-
 Corynebacterium acetophilum O-butyl-homoserine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + n-butanol
-
 Corynebacterium acetophilum A51 O-butyl-homoserine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + n-pentanol
-
 Corynebacterium acetophilum O-pentyl-homoserine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + n-propanol
-
 Corynebacterium acetophilum O-propyl-homoserine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + n-propanol
-
 Corynebacterium acetophilum A51 O-propyl-homoserine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-serine + H2S
-
 Corynebacterium acetophilum L-cysteine + acetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.49 dimer 2 * 110000, SDS-PAGE Corynebacterium acetophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.49 42 45
-
 Corynebacterium acetophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 8.5
-
-
 Corynebacterium acetophilum

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate tightly bound Corynebacterium acetophilum