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Literature summary extracted from

  • Kerr, D.S.
    O-Acetylhomoserine sulfhydrylase from Neurospora. Purification and consideration of its function in homocysteine and methionine synthesis (1971), J. Biol. Chem., 246, 95-102.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.49 enzyme remains stable after purification, no dilution inactivation is observed Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 L-methionine
-
 Neurospora crassa
2.5.1.49 S-adenosyl-L-homocysteine
-
 Neurospora crassa
2.5.1.49 S-adenosyl-L-methionine
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 0.7
-
 H2S
-
 Neurospora crassa
2.5.1.49 0.8
-
 methyl-mercaptan
-
 Neurospora crassa
2.5.1.49 7
-
 O-acetyl-L-homoserine
-
 Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + H2S Neurospora crassa
-
 L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methanethiol Neurospora crassa pathway for formation of methionine, incorporation of sulfur, possible temporary function for this enzyme may be formation of methionine from methylmercaptan at times of exhaustion of an exogenous source of sulfur L-methionine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Neurospora crassa
-
 wild-type, strain EM 5297
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 4
-
-
 Neurospora crassa
2.5.1.49 4.2
-
 O-acetylhomoserine sulfhydrylation Neurospora crassa
2.5.1.49 7.7
-
 O-acetylhomoserine methylsulfhydrylation Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
2.5.1.49 -15°C, 80% of the activity remains after storage for 6 months Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 additional information no cystathione formed from cysteine, no alpha-ketobutyrate formed from O-acetylhomoserine, negligible activity with homoserine and serine, O-succinylhomoserine, O-phosphorylhomoserine, O-acetylserine and O-phosphorylserine are no substrates Neurospora crassa ?
-
 ?
2.5.1.49 O-acetyl-L-homoserine + H2S
-
 Neurospora crassa L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methanethiol
-
 Neurospora crassa L-methionine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methanethiol pathway for formation of methionine, incorporation of sulfur, possible temporary function for this enzyme may be formation of methionine from methylmercaptan at times of exhaustion of an exogenous source of sulfur Neurospora crassa L-methionine + acetate
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methyl mercaptan
-
 Neurospora crassa L-methionine + acetic acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 8
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.49 6.6 8.5 50% activity at pH 6.6, 90% activity at pH 7.5 and 8.5 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate
-
 Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.49 0.01
-
 S-adenosylmethionine
-
 Neurospora crassa