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Literature summary extracted from

  • Watson, D.; Jourdian, G.W.; Roseman, S.
    The sialic acids. VIII. Sialic acid 9-phosphate synthetase (1966), J. Biol. Chem., 241, 5627-5636.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.57 purified enzyme is unstable to freezing and thawing Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.57 EDTA
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.57 0.69
-
 N-acetyl-D-mannosamine 6-phosphate
-
 Sus scrofa
2.5.1.57 1.6
-
 N-glycolyl-D-mannosamine 6-phosphate
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.57 Fe2+
-
 Rattus norvegicus
2.5.1.57 Fe2+
-
 Sus scrofa
2.5.1.57 Mg2+
-
 Rattus norvegicus
2.5.1.57 Mg2+ essential, 0.01 M required for maximal activity Sus scrofa
2.5.1.57 Mn2+
-
 Rattus norvegicus
2.5.1.57 Mn2+
-
 Sus scrofa
2.5.1.57 additional information Ca2+ or Cu2+ does not substitute for Mg2+ Rattus norvegicus
2.5.1.57 additional information Ca2+ or Cu2+ does not substitute for Mg2+ Sus scrofa
2.5.1.57 additional information Al3+ does not substitute for Mg2+ Sus scrofa
2.5.1.57 Ni2+
-
 Rattus norvegicus
2.5.1.57 Ni2+
-
 Sus scrofa
2.5.1.57 Zn2+
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Homo sapiens
-
 N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Rattus norvegicus
-
 N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Sus scrofa
-
 N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Ovis aries
-
 N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.57 Homo sapiens
-
 human
-
2.5.1.57 Ovis aries
-
 sheep
-
2.5.1.57 Rattus norvegicus
-
 rat
-
2.5.1.57 Sus scrofa
-
 hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.57
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.57 adrenal gland
-
 Sus scrofa
-
2.5.1.57 blood
-
 Homo sapiens
-
2.5.1.57 brain
-
 Rattus norvegicus
-
2.5.1.57 brain
-
 Sus scrofa
-
2.5.1.57 erythrocyte
-
 Homo sapiens
-
2.5.1.57 erythrocyte
-
 Rattus norvegicus
-
2.5.1.57 heart
-
 Sus scrofa
-
2.5.1.57 kidney
-
 Rattus norvegicus
-
2.5.1.57 liver
-
 Rattus norvegicus
-
2.5.1.57 lung
-
 Rattus norvegicus
-
2.5.1.57 muscle
-
 Rattus norvegicus
-
2.5.1.57 muscle
-
 Sus scrofa
-
2.5.1.57 ovary
-
 Sus scrofa
-
2.5.1.57 skin
-
 Rattus norvegicus
-
2.5.1.57 spleen
-
 Rattus norvegicus
-
2.5.1.57 submaxillary gland
-
 Homo sapiens
-
2.5.1.57 submaxillary gland
-
 Sus scrofa
-
2.5.1.57 submaxillary gland
-
 Ovis aries
-
2.5.1.57 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.57 0.26
-
-
 Homo sapiens
2.5.1.57 0.83
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.5.1.57 -18°C, crude extract can be stored Sus scrofa
2.5.1.57 4°C, purified enzyme loses approximately half of its activity in 3 days Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Homo sapiens N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Rattus norvegicus N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Sus scrofa N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Ovis aries N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Sus scrofa N-acylneuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Homo sapiens N-acylneuraminic acid 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Sus scrofa N-acylneuraminic acid 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Ovis aries N-acylneuraminic acid 9-phosphate + phosphate
-
 ?
2.5.1.57 N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Homo sapiens N-glycolyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Sus scrofa N-glycolyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-glycolyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Ovis aries N-glycolyl-neuraminate 9-phosphate + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.57 7.8
-
-
 Sus scrofa