Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoshikawa, T.; Furuya, T.
    Purification and properties of sterol:UDPG glucosyltransferase in cell culture of Digitalis purpurea (1979), Phytochemistry, 18, 239-241.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.173 2-mercaptoethanol activated by Digitalis purpurea
2.4.1.173 DIECA activated by Digitalis purpurea
2.4.1.173 EDTA activated by Digitalis purpurea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.173 NH4+ inhibited by Digitalis purpurea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 microsome
-
 Digitalis purpurea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.173 Mn2+ inhibited by Digitalis purpurea
2.4.1.173 Zn2+ inhibited by Digitalis purpurea

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Digitalis purpurea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 partial purification by differential centrifugation and Sephadex G-100 column, DEAE-Sephadex A-50 and Sepharose6B Digitalis purpurea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 cell culture leaf cell culture Digitalis purpurea
-
2.4.1.173 leaf crude extracts have 2-5 times lower activity than cell cultures Digitalis purpurea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 additional information almost no activity with androsterone, digitoxigenin, digitoxin and testosterone Digitalis purpurea ?
-
 ?
2.4.1.173 UDP-glucose + cholesterol best substrate Digitalis purpurea UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + epiandrosterone best substrate Digitalis purpurea UDP + epiandrosterone 3-beta-glucoside
-
 ?
2.4.1.173 UDP-glucose + pregnenolone 57% of the activity with epiandrosterone Digitalis purpurea UDP + pregnenolone 3-beta-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol 88% of the activity with epiandrosterone Digitalis purpurea UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol 122% of the activity with epiandrosterone, highest specificity Digitalis purpurea UDP + stigmasterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Digitalis purpurea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.173 7.5
-
-
 Digitalis purpurea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 7.5
-
 Tris-HCl 0.1 M, EDTA 10 mM, 2-mercaptoethanol 1 mM, sodium-deoxycholate 0.1% Digitalis purpurea