EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.21 | expression of pncB gene on a multicopy pUC19 plasmid in Salmonella typhimurium | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.21 | 0.0015 | - |
nicotinate | pncB gene, in the coupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
6.3.4.21 | 0.022 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pncB gene, in the coupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
6.3.4.21 | 0.29 | - |
nicotinate | pncB gene, in the uncoupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
6.3.4.21 | 4.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pncB gene, in the uncoupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.3.4.21 | soluble | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.4.21 | 45000 | - |
pncB gene, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.21 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.21 | bacterial suspension disruption and centrifugation, chromatography on Sephadex-G, DEAE-Toyopearl, Cibacron Blue and Sephacryl gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.4.21 | 3 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Storage Stability | Organism |
---|---|---|
6.3.4.21 | 4ºC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT | Salmonella enterica subsp. enterica serovar Typhimurium |
6.3.4.21 | t1/2: at least 2 months | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.21 | nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.21 | monomer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.21 | NAPRTase | - |
- |
6.3.4.21 | nicotinate phosphoribosyltransferase | - |
- |
6.3.4.21 | nicotinic acid phosphoribosyltransferase | - |
- |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.21 | ATP | 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed | Salmonella enterica subsp. enterica serovar Typhimurium |