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Literature summary extracted from

  • Ohta, D.; Fujimori, K.; Mizutani, M.; Nakayama, Y.; Kunpaisal-Hashimoto, R.; Munzer, S.; Kozaki, A.
    Molecular cloning and characterization of ATP-phosphoribosyl transferase from Arabidopsis, a key enzyme in the histidine biosynthetic pathway (2000), Plant Physiol., 122, 907-914.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.17 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 1,2,4-Triazole-3-alanine
-
 Arabidopsis thaliana
2.4.2.17 L-histidine feed-back inhibition; feed-back inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.17 0.13
-
 5-phospho-alpha-D-ribose 1-diphosphate recombinant At-ATP-PRT1 Arabidopsis thaliana
2.4.2.17 0.51
-
 ATP crude cell extracts Arabidopsis thaliana
2.4.2.17 0.57
-
 5-phospho-alpha-D-ribose 1-diphosphate crude cell extracts Arabidopsis thaliana
2.4.2.17 0.6
-
 ATP recombinant enzyme Arabidopsis thaliana
2.4.2.17 37
-
 5-phospho-alpha-D-ribose 1-diphosphate crude cell extracts Arabidopsis thaliana
2.4.2.17 89
-
 ATP crude cell extracts Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.17 44600
-
 calculated mass from amino acid sequence Arabidopsis thaliana
2.4.2.17 44800
-
 calculated mass from amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Arabidopsis thaliana first step in histidine biosynthesis diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Arabidopsis thaliana
-
 At-ATP-PRT2
-
2.4.2.17 Arabidopsis thaliana
-
 ecotype Columbia
-
2.4.2.17 Arabidopsis thaliana Q9S762 At-ATP-PRT1
-
2.4.2.17 Arabidopsis thaliana Q9S762 ecotype Columbia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.17 the recombinant enzymes are produced as fused proteins with a maltose-binding protein, and the purification is made by a two step amylose resin column followed by a digestion with Factor Xa Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Arabidopsis thaliana 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate first step in histidine biosynthesis Arabidopsis thaliana diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.17 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.17 8.5
-
 assay at Arabidopsis thaliana