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Literature summary extracted from

  • Plaut, G.W.E.; Harvey, R.A.
    The enzymatic synthesis of riboflavin (1971), Methods Enzymol., 18B, 515-538.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.9 2-Amino-4,6-dihydroxy-8-D-ribityl-7-pteridinone
-
 Saccharomyces cerevisiae
2.5.1.9 5,6,7,8-Tetrahydro-9-(1'-D-ribityl)isoalloxazine
-
 Saccharomyces cerevisiae
2.5.1.9 6,7-dihydroxy-8-ribityllumazine
-
 Saccharomyces cerevisiae
2.5.1.9 6,7-dimethyl-8-(1'-D-xylityl)lumazine
-
 Saccharomyces cerevisiae
2.5.1.9 6-Methyl-7-hydroxy-8-ribityllumazine
-
 Saccharomyces cerevisiae
2.5.1.9 p-chloromercuribenzenesulfonate reversible by cysteine or 2-mercaptoethanol Saccharomyces cerevisiae
2.5.1.9 riboflavin product inhibition, Saccharomyces cerevisiae Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.9 70000 80000 analytical ultracentrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Lactiplantibacillus plantarum
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Bacillus subtilis
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Saccharomyces cerevisiae
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Neurospora crassa
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Klebsiella aerogenes
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Pseudomonas sp.
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Eremothecium gossypii
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.9 Bacillus subtilis
-
-
-
2.5.1.9 Eremothecium gossypii
-
-
-
2.5.1.9 Escherichia coli
-
-
-
2.5.1.9 Klebsiella aerogenes
-
-
-
2.5.1.9 Lactiplantibacillus plantarum
-
-
-
2.5.1.9 Neurospora crassa
-
-
-
2.5.1.9 Pseudomonas sp.
-
-
-
2.5.1.9 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.9
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Lactiplantibacillus plantarum
a
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Bacillus subtilis
a
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Escherichia coli
a
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Saccharomyces cerevisiae
a
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Neurospora crassa
a
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Klebsiella aerogenes
a
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Pseudomonas sp.
a
2.5.1.9 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Eremothecium gossypii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.9 additional information
-
-
 Bacillus subtilis
2.5.1.9 additional information
-
-
 Saccharomyces cerevisiae
2.5.1.9 additional information
-
-
 Eremothecium gossypii

Storage Stability

EC Number Storage Stability Organism
2.5.1.9 -90°C or in liquid N2, stable for several months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Lactiplantibacillus plantarum riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Bacillus subtilis riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Escherichia coli riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Saccharomyces cerevisiae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Neurospora crassa riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Klebsiella aerogenes riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Pseudomonas sp. riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Eremothecium gossypii riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-[1'-(5'-deoxy-D-ribityl)]lumazine + 6,7-dimethyl-8-[1'-(5'-deoxy-D-ribityl)]lumazine
-
 Saccharomyces cerevisiae 5'-deoxyriboflavin + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.9 37
-
 assay at Lactiplantibacillus plantarum
2.5.1.9 37
-
 assay at Bacillus subtilis
2.5.1.9 37
-
 assay at Escherichia coli
2.5.1.9 37
-
 assay at Saccharomyces cerevisiae
2.5.1.9 37
-
 assay at Neurospora crassa
2.5.1.9 37
-
 assay at Klebsiella aerogenes
2.5.1.9 37
-
 assay at Pseudomonas sp.
2.5.1.9 37
-
 assay at Eremothecium gossypii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.9 7
-
-
 Bacillus subtilis
2.5.1.9 7
-
-
 Escherichia coli
2.5.1.9 7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.9 5.8 8.4 about 50% of activity maximum at pH 5.8 and 8.4 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.9 4 8 0°C, stable for several h in presence of a suitable reducing agent Saccharomyces cerevisiae