Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schmitt, D.; Callan, K.; Gruissem.W.
    Molecular and biochemical characterization of tomato farnesyl-protein transferase (1996), Plant Physiol., 112, 767-777.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.58 expression in Escherichia coli Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.58 EDTA
-
 Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.58 Mg2+ required Solanum lycopersicum
2.5.1.58 Zn2+ required Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.58 100000
-
 gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.58 farnesyl diphosphate + protein-cysteine Solanum lycopersicum a possible role for the enzyme during fruit development S-farnesyl protein + diphosphate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.5.1.58 additional information EDTA-treatment decreases activity Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.58 Solanum lycopersicum
-
 4-week-old hydroponically grown tomato plants, VFNT cherry
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.58 recombinant enzyme Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.58 fruit decreased activity approximately 10fold between young and mature green fruits, increased activity during fruit development in red ripe fruit Solanum lycopersicum
-
2.5.1.58 leaf low activity Solanum lycopersicum
-
2.5.1.58 additional information no significant difference in enzyme activity between dark-grown and light-grown seedlings Solanum lycopersicum
-
2.5.1.58 root
-
 Solanum lycopersicum
-
2.5.1.58 stem high activity in tissues from young plants Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.58 0.08
-
-
 Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.58 farnesyl diphosphate + protein-cysteine the specific CAQQ motif of the ANJ1 protein has a low affinity for enzyme in vitro, enzyme specifically farnesylated the RasB-CVIM peptide substrate Solanum lycopersicum diphosphate + S-farnesyl protein
-
 ?
2.5.1.58 farnesyl diphosphate + protein-cysteine a possible role for the enzyme during fruit development Solanum lycopersicum S-farnesyl protein + diphosphate
-
 ?
2.5.1.58 farnesyl diphosphate + Ras-Cys-Val-Ile-Met best substrate Solanum lycopersicum diphosphate + Ras-S-farnesyl-Cys-Val-Ile-Met
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.58 heterodimer
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.58 30
-
-
 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.58 6.5 7
-
 Solanum lycopersicum