EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.58 | expression in Escherichia coli | Solanum lycopersicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.58 | EDTA | - |
Solanum lycopersicum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.58 | Mg2+ | required | Solanum lycopersicum | |
2.5.1.58 | Zn2+ | required | Solanum lycopersicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.58 | 100000 | - |
gel filtration | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.58 | farnesyl diphosphate + protein-cysteine | Solanum lycopersicum | a possible role for the enzyme during fruit development | S-farnesyl protein + diphosphate | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.5.1.58 | additional information | EDTA-treatment decreases activity | Solanum lycopersicum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.58 | Solanum lycopersicum | - |
4-week-old hydroponically grown tomato plants, VFNT cherry | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.58 | recombinant enzyme | Solanum lycopersicum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.58 | fruit | decreased activity approximately 10fold between young and mature green fruits, increased activity during fruit development in red ripe fruit | Solanum lycopersicum | - |
2.5.1.58 | leaf | low activity | Solanum lycopersicum | - |
2.5.1.58 | additional information | no significant difference in enzyme activity between dark-grown and light-grown seedlings | Solanum lycopersicum | - |
2.5.1.58 | root | - |
Solanum lycopersicum | - |
2.5.1.58 | stem | high activity in tissues from young plants | Solanum lycopersicum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.58 | 0.08 | - |
- |
Solanum lycopersicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.58 | farnesyl diphosphate + protein-cysteine | the specific CAQQ motif of the ANJ1 protein has a low affinity for enzyme in vitro, enzyme specifically farnesylated the RasB-CVIM peptide substrate | Solanum lycopersicum | diphosphate + S-farnesyl protein | - |
? | |
2.5.1.58 | farnesyl diphosphate + protein-cysteine | a possible role for the enzyme during fruit development | Solanum lycopersicum | S-farnesyl protein + diphosphate | - |
? | |
2.5.1.58 | farnesyl diphosphate + Ras-Cys-Val-Ile-Met | best substrate | Solanum lycopersicum | diphosphate + Ras-S-farnesyl-Cys-Val-Ile-Met | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.58 | heterodimer | - |
Solanum lycopersicum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.58 | 30 | - |
- |
Solanum lycopersicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.58 | 6.5 | 7 | - |
Solanum lycopersicum |