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Literature summary extracted from

  • Takahashi, I.; Ogura, K.
    Prenyltransferases of Bacillus subtilis: undecaprenyl pyrophosphate synthetase and geranylgeranyl pyrophosphate synthetase (1982), J. Biochem., 92, 1527-1537.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.29 Triton X-100
-
 Bacillus subtilis
2.5.1.29 Tween 80 stimulates Bacillus subtilis
2.5.1.31 polyamines stimulate in presence of Mg2+ Bacillus subtilis
2.5.1.31 putrescine stimulates Bacillus subtilis
2.5.1.31 Triton X-100
-
 Bacillus subtilis
2.5.1.31 Tween 80 stimulates Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.29 NH4+ 20 mM, 20% inhibition Bacillus subtilis
2.5.1.31 NH4+ 20 mM, 20% inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 0.0133
-
 geranyl diphosphate
-
 Bacillus subtilis
2.5.1.31 0.0091
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0091
-
 (E,E)-farnesyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0093
-
 (2E,6E,10E)-geranylgeranyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0093
-
 (E,E,E)-geranylgeranyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0154
-
 (2Z,6E,10E)-geranylgeranyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0154
-
 (Z,E,E)-Geranylgeranyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0189
-
 (Z,E)-farnesyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0267
-
 neryl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0526
-
 (Z,Z,E,E)-farnesylgeranyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0526
-
 (2Z,6Z,10E,14E)-farnesylgeranyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0588
-
 (Z,E,E,E)-farnesylgeranyl diphosphate pH 8.5, 37°C Bacillus subtilis
2.5.1.31 0.0588
-
 (2Z,6E,10E,14E)-farnesylgeranyl diphosphate pH 8.5, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.29 Mg2+ required for activity Bacillus subtilis
2.5.1.29 Mg2+ optimal activity at 5 mM Bacillus subtilis
2.5.1.29 Mn2+ activates Bacillus subtilis
2.5.1.29 Mn2+ optimal activity at 0.5 mM Bacillus subtilis
2.5.1.31 K+ stimulates in presence of Mg2+ Bacillus subtilis
2.5.1.31 Mg2+ divalent cation required Bacillus subtilis
2.5.1.31 Mg2+ optimal activity at 5 mM, required Bacillus subtilis
2.5.1.31 Mn2+ can partially replace Mg2+ Bacillus subtilis
2.5.1.31 Mn2+ can partially replace Mg2+, optimal activity at 0.5 mM Bacillus subtilis
2.5.1.31 Na+ stimulates in presence of Mg2+ Bacillus subtilis
2.5.1.31 NH4+ stimulates in presence of Mg2+ Bacillus subtilis
2.5.1.31 Zn2+ can partially replace Mg2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 85000
-
 gel filtration Bacillus subtilis
2.5.1.31 57000
-
 gel filtration Bacillus subtilis
2.5.1.31 85000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Bacillus subtilis
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate Bacillus subtilis
-
 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Bacillus subtilis
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Bacillus subtilis
-
-
-
2.5.1.31 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 partial Bacillus subtilis
2.5.1.31 partial Bacillus subtilis
2.5.1.31 partially purified Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.31 0.000424
-
-
 Bacillus subtilis
2.5.1.31 0.000424
-
 pH 8.5, 37°C Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.5.1.31 0°C, 24 h, 56% loss of activity, partially purified enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis geranylgeranyl diphosphate + diphosphate E,E)-geranylgeranyl diphosphate ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Bacillus subtilis geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Bacillus subtilis geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Bacillus subtilis geranylgeranyl diphosphate + 2 diphosphate E)-farnesyl diphosphate + (E,E,E)-geranylgeranyl diphosphate ?
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Bacillus subtilis 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
2.5.1.31 (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?
2.5.1.31 (2Z,6E)-famesyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?
2.5.1.31 (2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?
2.5.1.31 (2Z,6E,10E,14E)-farnesylgeranyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?
2.5.1.31 (2Z,6Z,10E,14E)-farnesylgeranyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?
2.5.1.31 (Z,E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?
2.5.1.31 (Z,E,E,E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (E,E)-farnesyl diphosphate or (Z,E)-farnesyl diphosphate Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 3:1 from (Z,E,E,E)-farnesylgeranyl diphosphate and in the ratio 3:2 from (Z,Z,E,E)-farnesylgeranyl diphosphate ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (E,E)-farnesyl diphosphate or (Z,E)-farnesyl diphosphate Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate C50-polyprenyl diphosphate and C55-polyprenyl diphosphate with Z,E mixed stereochemistry ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (E,E)-farnesyl diphosphate or (Z,E)-farnesyl diphosphate Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate formation of C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 1:4 from (E,E)-farnesyl diphosphate and in the ratio of 4:1 from (Z,E)-farnesyl diphosphate ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (Z,E,E,E)-farnesylgeranyl diphosphate or (Z,Z,E,E)-farnesylgeranyl diphosphate Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 3:1 from (Z,E,E,E)-farnesylgeranyl diphosphate and in the ratio 3:2 from (Z,Z,E,E)-farnesylgeranyl diphosphate ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (Z,E,E,E)-farnesylgeranyl diphosphate or (Z,Z,E,E)-farnesylgeranyl diphosphate Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate C50-polyprenyl diphosphate and C55-polyprenyl diphosphate with Z,E mixed stereochemistry ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (Z,E,E,E)-farnesylgeranyl diphosphate or (Z,Z,E,E)-farnesylgeranyl diphosphate Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate formation of C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 1:4 from (E,E)-farnesyl diphosphate and in the ratio of 4:1 from (Z,E)-farnesyl diphosphate ?
2.5.1.31 isopentenyl diphosphate + geranyl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate (E,E,E)-geranylgeranyl diphosphate or (Z,E,E)-geranylgeranyl diphosphate Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate C50-prenyl diphosphate and C55-prenyl diphosphate in the ratio of 2:3 from (E,E,E)-geranyl diphosphate and in the ratio 1:3 from (Z,E,E)-geranylgeranyl diphosphate ?
2.5.1.31 isopentenyl diphosphate + neryl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate C45 prenyl diphosphate, C50 prenyl diphosphate and C55 prenyl diphosphate in the ratio of 2:10:1 ?
2.5.1.31 neryl diphosphate + isopentenyl diphosphate
-
 Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.31 undecaprenyl pyrophosphate synthetase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.31 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.29 8.5
-
-
 Bacillus subtilis
2.5.1.31 8.5
-
-
 Bacillus subtilis
2.5.1.31 8.5
-
 assay at Bacillus subtilis