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Literature summary extracted from

  • Ravanel, S.; Droux, M.; Douce, R.
    Methionine biosynthesis in higher plants. I. Purification and characterization of cystathionine gamma-synthase from spinach chloroplasts (1995), Arch. Biochem. Biophys., 316, 572-584.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.48 propargylglycine
-
Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.48 0.18
-
L-cysteine pH 7.5, 30°C Spinacia oleracea
2.5.1.48 0.6
-
S2- pH 7.5, 30°C Spinacia oleracea
2.5.1.48 1.4
-
O-phospho-L-homoserine pH 7.5, 30°C Spinacia oleracea
2.5.1.48 13.34
-
O-succinyl-L-homoserine pH 7.5, 30°C Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.48 chloroplast
-
Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.48 50000
-
4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea
2.5.1.48 53000
-
4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea
2.5.1.48 215000
-
gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.48 Spinacia oleracea
-
spinach
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.5.1.48 proteolytic modification the 50000 Da polypeptide may be derived from the 53000 Da polypeptide by proteolysis Spinacia oleracea

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.48
-
Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.48 leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.48 13.11
-
-
Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.5.1.48 -20°C, 20 mM MOPS-NaOH, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% v/v glycerol, 5 days, 50% loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.48 L-cystathionine + H2O
-
Spinacia oleracea L-homocysteine + NH3 + pyruvate
-
?
2.5.1.48 O-phospho-L-homoserine + L-cysteine
-
Spinacia oleracea L-cystathionine + phosphate
-
?
2.5.1.48 O-phospho-L-homoserine + S2-
-
Spinacia oleracea L-homocysteine + phosphate
-
?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine
-
Spinacia oleracea L-cystathionine + succinate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.48 tetramer 4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.48 7.4
-
-
Spinacia oleracea