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Literature summary extracted from

  • Litzenberger Holbrook, E.; Greene, R.C.; Heilig Krueger, J.
    Purification and properties of cystathionine gamma-synthase from overproducing strains of Escherichia coli (1990), Biochemistry, 29, 435-442.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.48 0.33
-
 O-succinyl-L-homoserine pH 8.2, 25°C, gamma-elimination reaction Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.48 39000
-
 4 * 39000, SDS-PAGE Escherichia coli
2.5.1.48 41503
-
 4 * 41503, calculation from sequence of DNA Escherichia coli
2.5.1.48 160000
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 160000
-
 sedimentation equilibrium centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.48 Escherichia coli
-
-
-
2.5.1.48 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.48
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.48 9
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.48 O-succinyl-L-homoserine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium succinate + 2-oxobutyrate + NH3
-
 ?
2.5.1.48 O-succinyl-L-homoserine + H2O
-
 Escherichia coli succinate + 2-oxobutyrate + NH3
-
 ?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-cystathionine + succinate
-
 ?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine
-
 Escherichia coli L-cystathionine + succinate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.48 tetramer 4 * 39000, SDS-PAGE Escherichia coli
2.5.1.48 tetramer 4 * 41503, calculation from sequence of DNA Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.48 7.67
-
 cystathionine pH 8.2, 25°C, 460 mol product formed per mol of subunit Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.48 pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 pyridoxal 5'-phosphate 4 mol per tetrameric holoenzyme Escherichia coli