Literature summary extracted from

Kanzaki, H.; Kobayashi, M.; Nagasawa, T.; Yamada, H.
Purification and characterization of cystathionine gamma-synthase type II from Bacillus sphaericus (1987), Eur. J. Biochem., 163, 105-112.

Inhibitors

EC Number	Inhibitors	Comment	Organism
2.5.1.48	3-Methyl-2-benzothiazolinone hydrazone	-	Lysinibacillus sphaericus
2.5.1.48	D-cycloserine	-	Lysinibacillus sphaericus
2.5.1.48	hydroxylamine	-	Lysinibacillus sphaericus
2.5.1.48	additional information	not inhibited by p-chloromercuribenzoate, iodoacetate, N-ethylmaleimide, 5,5'-dithiobis(2-nitrobenzoate), NaCN	Lysinibacillus sphaericus
2.5.1.48	phenylhydrazine	-	Lysinibacillus sphaericus
2.5.1.48	Semicarbazide	-	Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.5.1.48	43000	-	4 * 43000, SDS-PAGE	Lysinibacillus sphaericus
2.5.1.48	165000	-	gel filtration	Lysinibacillus sphaericus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.5.1.48	Lysinibacillus sphaericus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.5.1.48	-	Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.5.1.48	118	-	-	Lysinibacillus sphaericus

Storage Stability

EC Number	Storage Stability	Organism
2.5.1.48	-20°C, 10 mM potassium phosphate buffer, pH 7.5, 50% glycerol	Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.5.1.48	additional information	beta-elimination and gamma-elimination barely catalyzed	Lysinibacillus sphaericus	?	-	?
2.5.1.48	O-acetyl-L-homoserine + 2-mercaptoethanol	150% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-hydroxyethyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + 2-mercaptopropionate	50% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-methylcarboxymethyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + 3-mercaptopropionate	65% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-carboxyethyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + allyl mercaptan	4% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-allyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + benzyl mercaptan	41% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-benzyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + CH3SH	11% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	L-methionine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + D-Cys	101% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	D-allocystathionine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + D-homocysteine	125% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	meso-homolanthionine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + ethyl mercaptan	24% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	L-thionine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + isobutyl mercaptan	13% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-isobutyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + isopropyl mercaptan	1% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-isopropyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + L-Cys ethyl ester	15% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-[(2R)-2-amino-3-ethoxy-3-oxopropyl]-L-homocysteine + acetate	i.e. L-cystathionine monoethyl ester + acetate	?
2.5.1.48	O-acetyl-L-homoserine + L-Cys methyl ester	20% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-(L-2-amino-2-methoxycarbonyl)-L-homocysteine + acetate	i.e. L-cystathionine monomethyl ester + acetate	?
2.5.1.48	O-acetyl-L-homoserine + L-cysteine	-	Lysinibacillus sphaericus	L-cystathionine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + L-homocysteine	92% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	L-homolanthionine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + m-thiocresol	150% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-m-tolyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + N-acetyl-L-Cys	12% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-(L-acetylamino-2-carboxyethyl)-L-homocysteine + acetate	i.e. mono-N-acetyl-L-cystathionine + acetate	?
2.5.1.48	O-acetyl-L-homoserine + n-butyl mercaptan	5% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-n-butyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + n-propyl mercaptan	13% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-n-propyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + o-thiocresol	3% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-o-tolyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + p-nitrothiophenol	41% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-p-nitrophenyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + p-thiocresol	25% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-p-tolyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + phenyl mercaptan	98% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-phenyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + S2-	163% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + sec-butyl mercaptan	5% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-sec-butyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + tert-butyl mercaptan	2% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-tert-butyl-L-homosysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + thioacetic acid	3% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-acetyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + thioglycolate ethyl ester	348% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-ethoxycarbonyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + thioglycolate n-butyl ester	274% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-n-butoxycarbonyl-L-homocysteine + acetate	-	?
2.5.1.48	O-acetyl-L-homoserine + thiosalicylic acid	63% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	S-o-carboxyphenyl-L-homocysteine + acetate	-	?
2.5.1.48	O-malonyl-L-homoserine + L-cysteine	14% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	L-cystathionine + malonate	-	?
2.5.1.48	O-phenyl-L-homoserine + L-cysteine	1% relative activity to O-acetyl-L-homoserine + L-cysteine	Lysinibacillus sphaericus	L-cystathionine + phenol	-	?
2.5.1.48	O-succinyl-L-homoserine + L-cysteine	-	Lysinibacillus sphaericus	L-cystathionine + succinate	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.5.1.48	tetramer	4 * 43000, SDS-PAGE	Lysinibacillus sphaericus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.5.1.48	40	-	-	Lysinibacillus sphaericus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.5.1.48	7.5	-	-	Lysinibacillus sphaericus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.5.1.48	pyridoxal 5'-phosphate	4 mol per mol of enzyme, Km: 0.021 mM	Lysinibacillus sphaericus