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Literature summary extracted from

  • Taylor, W.P.; Sheflyan, G.Y.; Woodard, R.W.
    A single point mutation in 3-deoxy-D-manno-octulosonate-8-phosphate synthase is responsible for temperature sensitivity in a mutant strain of Salmonella typhimurium (2000), J. Biol. Chem., 275, 32141-32146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 enzyme from wild-type strain AG701 and temperature-sensitive strain AG701i50 expressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.55 P145S natural mutation in the temperature-sensitive strain AG701i50 leads to an increase in Km-value for both substrates, D-arabinose 5-phosphate and phosphoenolpyruvate, this mutant enzyme also has an altered oligomeric state. Reduced activity, about 35% of the wild-type, between 15 and 30°C. Above 30°C the activity of the mutant enzyme decreases dramatically Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 0.0028
-
 phosphoenolpyruvate pH 7.4, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 0.0042
-
 phosphoenolpyruvate pH 7.4, 37°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 0.0044
-
 D-arabinose 5-phosphate pH 7.4, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 0.0093
-
 D-arabinose 5-phosphate pH 7.4, 37°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 0.014
-
 D-arabinose 5-phosphate pH 7.4, 25°C, mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 0.26
-
 phosphoenolpyruvate pH 7.4, 25°C, mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 0.28
-
 D-arabinose 5-phosphate pH 7.4, 37°C, mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 1.65
-
 phosphoenolpyruvate pH 7.4, 37°C, mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.55 additional information
-
 two poorly resolved peaks are detected by gel filtration of the mutant enzyme P145S: 91000 Da and 53000 Da Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 97000
-
 wild-type enzyme, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Salmonella enterica subsp. enterica serovar Typhimurium
-
 wild-type strain AG701 and temperature-sensitive strain AG701i50
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55 recombinant enzyme from wild-type strain AG701 and temperature-sensitive strain AG701i50 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 trimer
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.55 37
-
 1 h, wild-type enzyme retains full activity, 50% loss of activity of the mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.55 0.72
-
 phosphoenolpyruvate pH 7.4, 25°C, mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 0.74
-
 D-arabinose 5-phosphate pH 7.4, 25°C, mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 1.63
-
 D-arabinose 5-phosphate pH 7.4, 37°C, mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 1.75
-
 D-arabinose 5-phosphate pH 7.4, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 2.02
-
 phosphoenolpyruvate pH 7.4, 37°C, mutant enzyme P145S Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 2.08
-
 phosphoenolpyruvate pH 7.4, 25°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 5.6
-
 D-arabinose 5-phosphate pH 7.4, 37°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.55 6.7
-
 phosphoenolpyruvate pH 7.4, 37°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium