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Literature summary extracted from

  • Leon, J.; Vega, J.M.
    Separation and regulatory properties of O-acetyl-L-serine sulfhydrylase isoenzymes from Chlamydomonas reinhardtii (1991), Plant Physiol. Biochem., 29, 595-599.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 Aminooxyacetate 57% and 64% inhibition at 1 mM, isoenzymes 1 and 2, respectively Chlamydomonas reinhardtii
2.5.1.47 hydroxylamine 35% and 48% inhibition at 5 mM, isoenzymes 1 and 2, respectively Chlamydomonas reinhardtii
2.5.1.47 L-cysteine 50% inhibition at 5 mM, only isoenzyme 1 Chlamydomonas reinhardtii
2.5.1.47 methionine 46% and 37% inhibition at 1 mM, isoenzymes 1 and 2, respectively Chlamydomonas reinhardtii
2.5.1.47 O-acetylserine
-
 Chlamydomonas reinhardtii
2.5.1.47 p-chloromercuribenzoate 14% and 4% inhibition at 1 mM, isoenzymes 1 and 2, respectively Chlamydomonas reinhardtii
2.5.1.47 Sulfide
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 5.2
-
 H2S pH 7.5, 50°C, isoenzyme 1 and 2 Chlamydomonas reinhardtii
2.5.1.47 9.6
-
 O-acetyl-L-serine pH 7.5, 50°C, isoenzyme 1 and 2 Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Chlamydomonas reinhardtii
-
 two isoenzymes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 15.4
-
 isoenzyme 2 Chlamydomonas reinhardtii
2.5.1.47 336
-
 isoenzyme 1 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine
-
 Chlamydomonas reinhardtii L-cysteine + acetate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 50
-
-
 Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 8.5
-
-
 Chlamydomonas reinhardtii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.47 6.5
-
 L-methionine pH 7.5, 50 C Chlamydomonas reinhardtii